| Differentiate RNA single-stranded region of the branched structures and hairpin loops by an octahedral cobalt(II) complex | |
|---|---|
| 學年 | 94 |
| 學期 | 1 |
| 出版（發表）日期 | 2005-12-01 |
| 作品名稱 | Differentiate RNA single-stranded region of the branched structures and hairpin loops by an octahedral cobalt(II) complex |
| 作品名稱（其他語言） | |
| 著者 | 鄭建中; Cheng, Chien-chung, Hung, Kuo-chan; Huang-Fu, Wei-chun |
| 單位 | 淡江大學化學學系 |
| 出版者 | 中國化學會 |
| 著錄名稱、卷期、頁數 | Journal of the Chinese Chemical Society 52(6), pp.1291-1297 |
| 摘要 | Single-stranded RNA molecules usually include secondary structural elements such as bulges, internal loops, and hairpin loops. These RNA secondary structural elements are often essential for the biological activity and functions of the RNA molecule. Chemical probe CoII(HAPP)(TFA)2 in the presence of H2O2 is found to differentiate single-stranded RNA from branched structures and hairpin loops. This study uses CoII(HAPP)(TFA)2 to analyze the structures of two RNA molecules: a fragment of HIV TAR RNA (TAR-27) and the catalytic domain 5 of group II intron (D5-29). The electrophoretic mobility of TAR-27 does not shift in the presence of CoII(HAPP)(TFA)2, suggesting that the reagent does not change the conformation of RNA substrate. Cleavage of the RNA substrates by CoII(HAPP)(TFA)2 unambiguously differentiated RNA internal looped structures from hairpin loops. The results show that CoII(HAPP)(TFA)2 is a sensitive, informative and convenient tool for analysis of RNA secondary structures. |
| 關鍵字 | |
| 語言 | en |
| ISSN | 0009-4536 |
| 期刊性質 | 國內 |
| 收錄於 | |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 否 |
| 國別 | TWN |
| 公開徵稿 | |
| 出版型式 | ,電子版 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/24931 ) |
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