教師資料查詢 | 類別: 期刊論文 | 教師: 陳曜鴻 CHEN, YAU-HUNG (瀏覽個人網頁)

標題:Multiple regulatory elements mediating neuronal-specific expression of zebrafish sodium channel gene, scn8aa
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學期
出版(發表)日期2008/09/01
作品名稱Multiple regulatory elements mediating neuronal-specific expression of zebrafish sodium channel gene, scn8aa
作品名稱(其他語言)
著者Wu, Si-huei; 陳曜鴻; Chen, Yau-hung; Huang, Fong-lee; Chang, Chuan-hsiung; Chang, Yi-feng; Tsay, Huey-jen
單位淡江大學化學學系
出版者Wiley-Blackwell
著錄名稱、卷期、頁數Developmental Dynamics 237(9), pp.2554-2565
摘要Zebrafish scn8aa sodium channels mediate the majority of sodium conductance, which is essential for the embryonic locomotor activities. Here, we investigated the transcriptional regulation of scn8aa in developing zebrafish embryos by constructing a GFP reporter driven by a 15-kb fragment of scn8aa gene designed as scn8aa:GFP. GFP expression patterns of scn8aa:GFP temporally and spatially recapitulated the expression of endogenous scn8aa mRNA during zebrafish embryonic development, with one exception in the inner nuclear layer of the retina. Three novel elements, along with an evolutionarily conserved element shared with mouse SCN8A, modulated neuronal-specific expression of scn8aa. The deletion of each positive element reduced the expression levels in neurons without inducing ectopic GFP expression in non-neuronal cells. Our results demonstrate that these four regulatory elements function cooperatively to enhance scn8aa expression in the zebrafish nervous system.
關鍵字
語言英文(美國)
ISSN1058-8388
期刊性質國外
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產學合作
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審稿制度
國別美國
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