Apoptosis of human melanoma cells induced by the novel compounds propolin A and propolin B from Taiwenese propolis
學年 95
學期 1
出版（發表）日期 2007-01-08
作品名稱 Apoptosis of human melanoma cells induced by the novel compounds propolin A and propolin B from Taiwenese propolis
作品名稱（其他語言）
著者 吳嘉麗; Wu, Chia-li; Chen, Chia-nan; Lin, Jen-kun
單位 淡江大學化學學系
出版者 Elsevier Ireland
著錄名稱、卷期、頁數 Cancer Letters 245(1-2), pp.218-231
摘要 We recently demonstrated that two new prenylflavanones, propolin A and propolin B, isolated and characterized from Taiwanese propolis, induced cytotoxicity effect in human melanoma A2058 cells and shows a strong capability to scavenge free radicals. In this study, propolin A effectively induced a cytotoxic effect on five different cancer cell lines. Similar results were obtained for propolin B. DNA flow cytometric analysis and DNA fragmentation ladder indicated that propolin A and propolin B actively induced apoptosis in A2058 cells. To address the mechanism of the apoptosis effect of propolin A and propolin B, we evaluated the apoptosis-related proteins in A2058 cells. The levels of procaspase-8, Bid, procaspase-3, DFF45, and PARP were decreased in dose- and time course-dependent manners. Furthermore, also found propolin A and propolin B was capable of releasing cytochrome c from mitochondria to cytosol. The findings suggest that propolin A and propolin B may activate a mitochondria-mediated apoptosis pathway. On the other hand, our data show that propolin B inhibitied xanthine oxidase activity more efficiently than propolin A or CAPE. However, CAPE suppressed ROS-induced DNA strand breakage more efficiently than propolin A or propolin B. All these results indicated that propolin A and propolin B may trigger apoptosis of A2058 cells through mitochondria-dependent pathways and also shown that propolin A and propolin B were strong antioxidants.
關鍵字 Propolin A;Propolin B;Taiwanese propolis;Apoptosis;Xanthine oxidase;Human melanoma cells
語言 en
ISSN 0304-3835
期刊性質 國內
收錄於
產學合作
通訊作者
審稿制度 否
國別 TWN
公開徵稿
出版型式 ,電子版
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