| A Fluorescent Carbazole Derivative: High Sensitivity for Quadruplex DNA | |
|---|---|
| 學年 | 92 |
| 學期 | 1 |
| 出版（發表）日期 | 2003-10-04 |
| 作品名稱 | A Fluorescent Carbazole Derivative: High Sensitivity for Quadruplex DNA |
| 作品名稱（其他語言） | |
| 著者 | Chang, C. C.; Wu, J. Y.; Chien, C. W.; Wu, W. S.; Liu, H.; Kang, C. C.; 余良杰; Yu, Liang-jye; Chang, D. C. |
| 單位 | 淡江大學化學學系 |
| 出版者 | American Chemical Society (ACS) |
| 著錄名稱、卷期、頁數 | Analytical Chemistry 75(22), pp.6177-6183 |
| 摘要 | We have synthesized a novel molecule, 3,6-bis(1-methyl-4-vinylpyridium)carbazole diiodide (BMVC), for recognizing specific quadruplex structures, particularly the quadruplex of human telomeric sequence d(T2AG3)4. The fluorescence intensity of the BMVC molecule increases from 1 to almost 2 orders of magnitude upon interacting with various DNAs. At a concentration of BMVC of 10 μM, fluorescence bands with different colors of BMVC in electrophoresis gels of various DNAs can be observed. The fluorescence of BMVC can be used to discriminate between duplex and quadruplex DNAs. At the low concentration of 0.1 μM BMVC in prestained gels, the fluorescence is observed in the presence of quadruplexes with anti−anti−anti−anti and anti−anti−syn−syn arrangements. However, no fluorescence band is detected upon interacting with duplexes and quadruplexes with anti−syn−anti−syn arrangement. Moreover, the sensitivity assays show that as little as 0.2 pmol of quadruplex of d(T2AG3)4 can be revealed by BMVC. |
| 關鍵字 | |
| 語言 | en_US |
| ISSN | 0003-2700 |
| 期刊性質 | 國外 |
| 收錄於 | |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 否 |
| 國別 | USA |
| 公開徵稿 | |
| 出版型式 | ,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/25001 ) |
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