期刊論文
| 學年 | 113 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 2025-06-17 |
| 作品名稱 | Production of monoclonal antibodies against carcinoembryonic antigen-related cell adhesion molecule 6 and detection of their binding affinities |
| 作品名稱（其他語言） | |
| 著者 | Yun-Hsin Wangb; Yau-Hung Chen |
| 單位 | |
| 出版者 | |
| 著錄名稱、卷期、頁數 | Tzu Chi Medical Journal 37(4), p.395-402 |
| 摘要 | Objectives: Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) is a glycophosphatidylinositol-anchored member of the immunoglobulin superfamily, often overexpressed in various malignancies. Targeting CEACAM6 by suppressing its expression can potentially reverse these effects, making it a promising therapeutic target. In this study, we generated five monoclonal antibodies (CEAS1, CEAS2, CEAS3, CEAS4, and CEAS5; CEAS1-S5) against the recombinant CEACAM6 protein. Materials and methods: Through enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) assay, we demonstrated that each antibody specifically binds to CEACAM6 without interfering with the binding of others. SPR analysis further revealed the rate of association (Ka), dissociation (Kd), and equilibrium dissociation constants (KD) for each antibody. Results: The KD values ranged from 5.089 × 10-11 to 1.213 × 10-13 M, with CEAS5 exhibiting the highest binding affinity. In addition, CEAS5, unlike CEAS1-S4, could bind to both CEACAM5 and CEACAM6, indicating its bivalent nature. Conclusion: These findings highlight the strong antigen-binding capabilities of CEAS1-S5, warranting further investigation. |
| 關鍵字 | Carcinoembryonic antigen-related cell adhesion molecule; Enzyme-linked immunosorbent assay; Monoclonal antibodies; Surface plasmon resonance |
| 語言 | en |
| ISSN | 1016-3190 |
| 期刊性質 | 國外 |
| 收錄於 | ESCI |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 否 |
| 國別 | IND |
| 公開徵稿 | |
| 出版型式 | ,電子版 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/129443 ) |