期刊論文
| 學年 | 113 |
|---|---|
| 學期 | 2 |
| 出版（發表）日期 | 2025-06-17 |
| 作品名稱 | Growth inhibition activity assessment of γ-lactam-containing chemicals in cultured MCF-7 cells and xenografted chick embryos |
| 作品名稱（其他語言） | |
| 著者 | Chiao-Yin Sun; Shu-Chun Cheng; Yun-Hsin Wang; Ching-Yuan Huang; Adam Shih-Yuan Lee; Tao-Sheng Li; Ta-Hsien Lin; Yau-Hung Chen |
| 單位 | |
| 出版者 | |
| 著錄名稱、卷期、頁數 | Journal of Biochemical and Molecular Toxicology 39(6), e70344 |
| 摘要 | We evaluated the growth-inhibitory effects of three γ-butyrolactam (γ-lactam) derivatives (compounds 5, 6, and 8) on human breast cancer cells (MCF-7) and in a xenografted chick model. Growth inhibition was assessed using MTT assays and flow cytometry for compounds 5, 6, and 8, followed by Western blot analysis to examine the expression levels of cell cycle-associated proteins. Chick embryos were utilized as a xenograft model for further validation. The results revealed that compound 8 exhibited relatively low toxicity among the tested compounds. Western blot analysis suggested that compounds 5, 6, and 8 may be involved in the EGFR and P53 signaling pathways. In the xenograft model, compound 8 significantly inhibited MCF-7 tumor growth in chick embryos (tumor size in control group: 1.97 ± 0.69 mm, n = 15; compound 8-treated group: 1.20 ± 0.37 mm, n = 15). These findings suggest that compound 8 effectively inhibits MCF-7 growth with minimal toxicity, warranting further investigation in mammalian models. |
| 關鍵字 | γ-lactams; butyrolactam; chick; MCF-7; xenograft |
| 語言 | en |
| ISSN | 1095-6670; 1099-0461 |
| 期刊性質 | 國外 |
| 收錄於 | SCI SSCI |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 是 |
| 國別 | USA |
| 公開徵稿 | |
| 出版型式 | ,電子版,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/129441 ) |