期刊論文
| 學年 | 114 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2025-09-01 |
| 作品名稱 | Protective effects of roselle (Hibiscus sabdariffa) aqueous extract against aristolochic acid-induced developmental nephrotoxicity in zebrafish |
| 作品名稱（其他語言） | |
| 著者 | Ping-Hsun Lu; Yun-Hsin Wang; Chi-Chung Wen; Yau-Hung Chen |
| 單位 | |
| 出版者 | |
| 著錄名稱、卷期、頁數 | Journal of Toxicological Pathology 38(4), p.213-221 |
| 摘要 | This study investigates the protective effects of roselle aqueous extract (RAE) against aristolochic acid (AA)-induced developmental nephrotoxicity in zebrafish embryos. Fluorescence imaging, immunohistochemistry, and real-time polymerase chain reaction (PCR) experiments were conducted. The results showed that pre-treatment with RAE significantly improved survival rates and reduced kidney malformations in AA-exposed zebrafish. Specifically, survival rates in the AA-treated group decreased to 81.11% by 72 hours post-fertilisation, whereas those in the AA+RAE (30 and 60 ppm) groups increased to 86.67–90%. Additionally, RAE pre-treatment reduced kidney malformation rates from 98.89% in the AA group to 75.56% and 63.33% in the AA+RAE (30 and 60 ppm) groups, respectively. Histological analysis revealed that RAE mitigated structural damage in the pronephric tubes, restoring their compact architecture. Molecular analysis using real-time reverse transcription (RT)-PCR further indicated that RAE reduced the expression of inflammatory markers, suggesting that its protective effects are mediated through the attenuation of AA-induced inflammation. These findings demonstrate that RAE exerts a protective role against AA-induced developmental kidney malformations in zebrafish, likely through its antioxidant and anti-inflammatory properties. |
| 關鍵字 | aristolochic acid; fluorescence; kidney; roselle; zebrafish |
| 語言 | en |
| ISSN | 1881-915X; 0914-9198 |
| 期刊性質 | 國外 |
| 收錄於 | SCI SSCI |
| 產學合作 | |
| 通訊作者 | |
| 審稿制度 | 是 |
| 國別 | JPN |
| 公開徵稿 | |
| 出版型式 | ,電子版,紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/129440 ) |