期刊論文
| 學年 | 100 |
|---|---|
| 學期 | 1 |
| 出版（發表）日期 | 2011-09-01 |
| 作品名稱 | Inhibition of the P2X7 Receptor Reduces Cystogenesis in PKD |
| 作品名稱（其他語言） | |
| 著者 | Chang, Ming-yang; Lu, Jenn-kan; Tian, Ya-chung; Chen, Yung-chang; Hung, Cheng-chieh; Huang, Yi-hui; Chen, Yau-hung; Wu, Mai-szu; Yang, Chih-wei; Cheng, Yi-chuan |
| 單位 | 淡江大學化學學系 |
| 出版者 | Washington: American Society of Nephrology |
| 著錄名稱、卷期、頁數 | Journal of the American Society of Nephrology 22(9), pp.1696-1706 |
| 摘要 | The P2X7 receptor participates in purinergic signaling, which may promote the progression of ADPKD. We examined the effects of a P2X7 receptor antagonist and a P2X7 receptor agonist on cyst development in a zebrafish model of polycystic kidney disease in which we knocked down pkd2 by morpholinos. We used live wt-1b pronephric-specific GFP-expressing zebrafish embryos to directly observe changes in the pronephros. Exposure of pkd2-morphant zebrafish to a P2X7 receptor antagonist (oxidized ATP [OxATP]) significantly reduced the frequency of the cystic phenotype compared with either exposure to a P2X7 receptor agonist (BzATP) or with no treatment (P ＜ 0.01). Histology confirmed improvement of glomerular cysts in OxATP-treated pkd2 morphants. OxATP also reduced p-ERK activity and cell proliferation in pronephric kidneys in pkd2 morphants. Inhibition of P2X7 with an additional specific antagonist (A-438079), and through morpholino-mediated knockdown of p2rx7, confirmed these effects. In conclusion, blockade of the P2X7 receptor reduces cyst formation via ERK-dependent pathways in a zebrafish model of polycystic kidney disease, suggesting that P2X7 antagonists may have therapeutic potential in ADPKD. |
| 關鍵字 | |
| 語言 | en_US |
| ISSN | 1046-6673 |
| 期刊性質 | 國外 |
| 收錄於 | SCI |
| 產學合作 | |
| 通訊作者 | Cheng, Yi-chuan |
| 審稿制度 | |
| 國別 | USA |
| 公開徵稿 | |
| 出版型式 | 紙本 |
| 相關連結 |
機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/77040 ) |