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摘要
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Chitosan is a biopolymer composed of glucosamine and N-acetyl glucosamine. It is produced by the deacetylation reaction of chitin, the second most abundant polysaccharide. The degree of deacetylation (DDA) of chitosan will affect its water solubility and, thus, its applications in biomedicine, the food industry, wastewater treatment, etc. The amino group of the glucosamine unit on the chitosan chain can provide the charge characteristics to be a solid adsorbent. In this study, we will explore the adsorption effects of different types of chitosan on ethidium bromide (EtBr), a positively charged DNA intercalating dye and, consequently, a carcinogenic reagent. We have investigated several factors that would affect the EtBr removal efficiency of chitosan adsorbent, including the chitosan DDA and molecular weights, the adsorption duration, the usage amount of chitosan adsorbent, and the EtBr concentration. We found that the removal efficiency could reach 60% by using 95%-DDA chitosan flakes as the adsorbent for the adsorption of 10 ppm EtBr solution for 24 hours, which was 10% higher than the result of the chitosan hydrogel beads under the same conditions. |
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