The angiogenesis-modulating effects of coumarin-derivatives
學年 112
學期 2
出版(發表)日期 2024-02-19
作品名稱 The angiogenesis-modulating effects of coumarin-derivatives
著者 Huang, Han-ting; Huang, Ching-yuan; Lee, Chih-jou; Sun, Bo-jie; Wen, Chi-chung; Wang, Yun-hsin; Chen, Yau-hung
著錄名稱、卷期、頁數 Comparative Biochemistry and Physiology Part C 278, 109862
摘要 Coumarin is a natural compound that is rich in plants. Coumarin and its derivates were reported to have many biological activities, such as anti-bacterial, anti-tumor, and anti-coagulation. In this study, we examined the angiogenic modulating activities of six previously synthesized coumarin derivatives (Compound #1-#6) in zebrafish embryos and further confirmed them in a chick model. According to the survival rate in a zebrafish model, Compound #1 (100 %), #2 (82.5–100 %), and #4 (100 %) showed much less toxicity than Compound #3 (19.2–100 %), #5 (0–100 %), and #6 (0–100 %). Using a green blood vessel fluorescent transgenic fish Tg(fli1:egfp) to record the angiogenesis-modulating effects of Compound #1, #2, and #4, we found that Compound #2 had the highest effects in interfering intersegmental vessel growth, subintestinal vein growth, and caudal vein plexus remodeling. Chick chorioallantoic membrane (CAM) assay also showed that Compound #2 exposure led to a reduction of blood vessel growth. Real-time PCR experiments revealed that Compound #2 significantly changed the expression of vascular growth-related genes flt1, cdh5, and nrp1a in zebrafish. Based on our data from zebrafish and chick models, a new coumarin-derivative (Compound #2) possesses anti-angiogenic activity with low toxicity, but further investigation in mammal models is asked to confirm our findings.
語言 en
ISSN 1878-1659
期刊性質 國外
收錄於 SCI
國別 USA
出版型式 ,電子版