In situ encapsulation of camptothecin by self-assembly of poly(acrylic acid)-b-poly(N-isopropylacrylamide) and chitosan for controlled drug delivery
學年 111
學期 2
出版（發表）日期 2023-05-26
作品名稱 In situ encapsulation of camptothecin by self-assembly of poly(acrylic acid)-b-poly(N-isopropylacrylamide) and chitosan for controlled drug delivery
作品名稱（其他語言）
著者 Yi-Cheng Huang; Yang-Jie Zeng; Yu-Wei Lin; Hung-Chih Tai; Trong-Ming Don
單位
出版者
著錄名稱、卷期、頁數 Polymers 15(11), 2463
摘要 Camptothecin (CPT) has been shown to exhibit anticancer activity against several cancers. Nevertheless, CPT is very hydrophobic with poor stability, and thus its medical application is limited. Therefore, various drug carriers have been exploited for effectively delivering CPT to the targeted cancer site. In this study, a dual pH/thermo-responsive block copolymer of poly(acrylic acid-b-N-isopropylacrylamide) (PAA-b-PNP) was synthesized and applied to encapsulate CPT. At temperatures above its cloud point, the block copolymer self-assembled to form nanoparticles (NPs) and in situ encapsulate CPT, owing to their hydrophobic interaction as evidenced by fluorescence spectrometry. Chitosan (CS) was further applied on the surface through the formation of a polyelectrolyte complex with PAA for improving biocompatibility. The average particle size and zeta potential of the developed PAA-b-PNP/CPT/CS NPs in a buffer solution were 168 nm and −30.6 mV, respectively. These NPs were still stable at least for 1 month. The PAA-b-PNP/CS NPs exhibited good biocompatibility toward NIH 3T3 cells. Moreover, they could protect the CPT at pH 2.0 with a very slow-release rate. At pH 6.0, these NPs could be internalized by Caco-2 cells, followed by intracellular release of the CPT. They became highly swollen at pH 7.4, and the released CPT was able to diffuse into the cells at higher intensity. Among several cancer cell lines, the highest cytotoxicity was observed for H460 cells. As a result, these environmentally-responsive NPs have the potential to be applied in oral administration.
關鍵字 stimuli-responsive polymers;poly(acrylic acid);poly(N-isopropylacrylamide);chitosan;camptothecin;drug delivery
語言 en
ISSN 2073-4360
期刊性質 國外
收錄於 SCI
產學合作
通訊作者 Trong-Ming Don
審稿制度 是
國別 SWE
公開徵稿
出版型式 ,電子版
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/124778 )