Novel α-amylase inhibitor hemi-pyocyanin produced by microbial conversion of chitinous discards
學年 110
學期 2
出版（發表）日期 2022-04-23
作品名稱 Novel α-amylase inhibitor hemi-pyocyanin produced by microbial conversion of chitinous discards
作品名稱（其他語言）
著者 Nguyen TH; Wang SL; Nguyen AD; Doan MD; Tran TN; Doan CT; Nguyen VB
單位
出版者
著錄名稱、卷期、頁數 Marine Drugs 20(5), p.283
摘要 α-Amylase inhibitors (aAIs) have been applied for the efficient management of type 2 diabetes. The aim of this study was to search for potential aAIs produced by microbial fermentation. Among various bacterial strains, Pseudomonas aeruginosa TUN03 was found to be a potential aAI-producing strain, and shrimp heads powder (SHP) was screened as the most suitable C/N source for fermentation. P. aeruginosa TUN03 exhibited the highest aAIs productivity (3100 U/mL) in the medium containing 1.5% SHP with an initial pH of 7–7.5, and fermentation was performed at 27.5 °C for two days. Further, aAI compounds were investigated for scaled-up production in a 14 L-bioreactor system. The results revealed a high yield (4200 U/mL) in a much shorter fermentation time (12 h) compared to fermentation in flasks. Bioactivity-guided purification resulted in the isolation of one major target compound, identified as hemi-pyocyanin (HPC) via gas chromatography-mass spectrometry and nuclear magnetic resonance. Its purity was analyzed by high-performance liquid chromatography. HPC demonstrated potent α-amylase inhibitory activity comparable to that of acarbose, a commercial antidiabetic drug. Notably, HPC was determined as a new aAI. The docking study indicated that HPC inhibits α-amylase by binding to amino acid Arg421 at the biding site on enzyme α-amylase with good binding energy (−9.3 kcal/mol) and creating two linkages of H-acceptors.
關鍵字 α-amylase inhibitors;diabetes;hemi-pyocyanin;marine discards;microbial conversion
語言 en_US
ISSN 1660-3397
期刊性質 國外
收錄於 SCI
產學合作
通訊作者
審稿制度 否
國別 USA
公開徵稿
出版型式 ,電子版
相關連結

機構典藏連結 ( http://tkuir.lib.tku.edu.tw:8080/dspace/handle/987654321/124221 )