| 標題：Potential of the liquid fermentation of fishery waste by Paenibacillus elgii for metalloprotease production. Polymers, 14(13): 2741. SCI https://www.mdpi.com/2073-4360/14/13/2741/pdf | |
|---|---|
| 學年 | 110 |
| 學期 | 2 |
| 出版（發表）日期 | 2022/07/05 |
| 作品名稱 | Potential of the liquid fermentation of fishery waste by Paenibacillus elgii for metalloprotease production. Polymers, 14(13): 2741. SCI https://www.mdpi.com/2073-4360/14/13/2741/pdf |
| 作品名稱（其他語言） | |
| 著者 | Doan CT, Tran TN, Nguyen MT, Nguyen HK, Tran TKT, Nguyen TH, Tran TPH, Nguyen VB, Nguyen AD, Wang SL* |
| 單位 | |
| 出版者 | |
| 著錄名稱、卷期、頁數 | Polymers, 14(13): 2741 |
| 摘要 | This study attempted to use fishery processing wastes to produce protease by Paenibacillus elgii
TKU051. Of the tested wastes, tuna head powder (THP) was found to be the most effective carbon and nitrogen (C/N) source, and the optimal conditions were as follows: 0.811% THP, 0.052% K2HPO4 , 0.073% MgSO4 , initial pH of 8.96, incubation temperature of 31.4 ◦C, and incubation time of 3.092 days to achieve the maximum protease activity of 2.635 ± 0.124 U/mL. A protease with a molecular weight of 29 kDa was purified and biochemically characterized. Liquid chromatography with tandem mass spectrometry analysis revealed an amino acid sequence of STVHYSTR of P. elgii TKU051 protease, suggesting that the enzyme may belong to the M4 family of metalloproteases. The optimal activity of the enzyme was achieved at 60 ◦C and pH 8. P. elgii TKU051 protease was strongly inhibited by ethylenediaminetetraacetic acid and 1,10-phenanthroline, indicating its precise metalloprotease property. P. elgii TKU051 protease displayed the activity toward casein and raw fishery wastes such as tuna heads, tuna viscera, shrimp heads, and squid pens. Finally, the purified P. elgii TKU051 protease could improve the free-radical scavenging activity of fishery wastes. In short, P. elgii TKU051 has potential application in eco-friendly approaches to efficiently convert fishery wastes to metalloprotease. |
| 關鍵字 | antioxidant; metalloprotease; fishery waste; Paenibacillus elgii; protease; tuna heads |
| 語言 | 中文 |
| ISSN | 2073-4360 |
| 期刊性質 | 國外 |
| 收錄於 | SCI; |
| 產學合作 | |
| 通訊作者 | WANG San-Lang |
| 審稿制度 | 1 |
| 國別 | 瑞士 |
| 公開徵稿 | |
| 出版型式 | ,電子版 |
| SDGs |
|
GoogleAnalytics
