教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎WANG SAN-LANG (瀏覽個人網頁)

標題:Potential of the liquid fermentation of fishery waste by Paenibacillus elgii for metalloprotease production. Polymers, 14(13): 2741. SCI https://www.mdpi.com/2073-4360/14/13/2741/pdf
學年110
學期2
出版(發表)日期2022/07/05
作品名稱Potential of the liquid fermentation of fishery waste by Paenibacillus elgii for metalloprotease production. Polymers, 14(13): 2741. SCI https://www.mdpi.com/2073-4360/14/13/2741/pdf
作品名稱(其他語言)
著者Doan CT, Tran TN, Nguyen MT, Nguyen HK, Tran TKT, Nguyen TH, Tran TPH, Nguyen VB, Nguyen AD, Wang SL*
單位
出版者
著錄名稱、卷期、頁數Polymers, 14(13): 2741
摘要This study attempted to use fishery processing wastes to produce protease by Paenibacillus elgii
TKU051. Of the tested wastes, tuna head powder (THP) was found to be the most effective carbon and
nitrogen (C/N) source, and the optimal conditions were as follows: 0.811% THP, 0.052% K2HPO4
,
0.073% MgSO4
, initial pH of 8.96, incubation temperature of 31.4 ◦C, and incubation time of 3.092 days
to achieve the maximum protease activity of 2.635 ± 0.124 U/mL. A protease with a molecular weight
of 29 kDa was purified and biochemically characterized. Liquid chromatography with tandem mass
spectrometry analysis revealed an amino acid sequence of STVHYSTR of P. elgii TKU051 protease,
suggesting that the enzyme may belong to the M4 family of metalloproteases. The optimal activity
of the enzyme was achieved at 60 ◦C and pH 8. P. elgii TKU051 protease was strongly inhibited
by ethylenediaminetetraacetic acid and 1,10-phenanthroline, indicating its precise metalloprotease
property. P. elgii TKU051 protease displayed the activity toward casein and raw fishery wastes such
as tuna heads, tuna viscera, shrimp heads, and squid pens. Finally, the purified P. elgii TKU051
protease could improve the free-radical scavenging activity of fishery wastes. In short, P. elgii
TKU051 has potential application in eco-friendly approaches to efficiently convert fishery wastes to
metalloprotease.
關鍵字antioxidant; metalloprotease; fishery waste; Paenibacillus elgii; protease; tuna heads
語言中文
ISSN2073-4360
期刊性質國外
收錄於SCI;
產學合作
通訊作者WANG San-Lang
審稿制度1
國別瑞士
公開徵稿
出版型式,電子版
SDGs
  • 消除貧窮,消除飢餓,良好健康和福祉,潔淨水與衛生
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