教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎WANG SAN-LANG (瀏覽個人網頁)

標題:Production of Sucrolytic Enzyme by Bacillus licheniformis by the Bioconversion of Pomelo Albedo as a Carbon Source
學年109
學期2
出版(發表)日期2021/06/13
作品名稱Production of Sucrolytic Enzyme by Bacillus licheniformis by the Bioconversion of Pomelo Albedo as a Carbon Source
作品名稱(其他語言)
著者Chien Thang Doan; Thi Ngoc Tran; Thi Thanh Nguyen; Thi Phuong Hanh Tran; Van Bon Nguyen; Trung Dung Tran; Anh Dzung Nguyen; San-Lang Wang
單位
出版者
著錄名稱、卷期、頁數Polymers 13(12), 1959
摘要Recently, there has been increasing use of agro-byproducts in microbial fermentation to
produce a variety of value-added products. In this study, among various kinds of agro-byproducts,
pomelo albedo powder (PAP) was found to be the most effective carbon source for the production of
sucrose hydrolyzing enzyme by Bacillus licheniformis TKU004. The optimal medium for sucrolytic
enzyme production contained 2% PAP, 0.75% NH4NO3
, 0.05% MgSO4
, and 0.05% NaH2PO4 and the
optimal culture conditions were pH 6.7, 35 ◦C, 150 rpm, and 24 h. Accordingly, the highest sucrolytic
activity was 1.87 U/mL, 4.79-fold higher than that from standard conditions using sucrose as the
carbon source. The purified sucrolytic enzyme (sleTKU004) is a 53 kDa monomeric protein and
belongs to the glycoside hydrolase family 68. The optimum temperature and pH of sleTKU004 were
50 ◦C, and pH = 6, respectively. SleTKU004 could hydrolyze sucrose, raffinose, and stachyose by
attacking the glycoside linkage between glucose and fructose molecules of the sucrose unit. The Km
and Vmax of sleTKU004 were 1.16 M and 5.99 µmol/min, respectively. Finally, sleTKU004 showed
strong sucrose tolerance and presented the highest hydrolytic activity at the sucrose concentration of
1.2 M–1.5 M.
關鍵字agro-byproduct;Bacillus licheniformis;bioconversion;pomelo albedo;sucrolytic
語言英文
ISSN2073-4360
期刊性質國外
收錄於SCI;
產學合作
通訊作者WANG San-Lang
審稿制度
國別瑞士
公開徵稿
出版型式,電子版
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