| 標題：Utilization of seafood processing by-products for production of proteases by Paenibacillus sp. TKU052 and their application in biopeptides' preparation |
|---|
| 學年 | 109 |
|---|
| 學期 | 1 |
|---|
| 出版（發表）日期 | 2020/11/20 |
|---|
| 作品名稱 | Utilization of seafood processing by-products for production of proteases by Paenibacillus sp. TKU052 and their application in biopeptides' preparation |
|---|
| 作品名稱（其他語言） | |
|---|
| 著者 | Doan CT; Tran TN; Nguyen VB; Nguyen AD; Wang SL |
|---|
| 單位 | |
|---|
| 出版者 | |
|---|
| 著錄名稱、卷期、頁數 | Marine Drugs 18(11), 574 |
|---|
| 摘要 | Microbial fermentation of by-products is a renewable and efficient technique in the development of a range of useful products. In this study, protease synthesis by Paenibacillus sp. TKU052 was carried out on culture media containing some common seafood processing by-products (SPBPs) as the sole source of carbon and nitrogen (C/N). The most suitable C/N nutrition source for the production of proteases was found to be 3.0% (w/v) demineralized crab shells powder (deCSP) and maximal enzyme activity of 4.41 ± 0.16 U/mL was detected on the third day of the culture. Two proteases (P1 and P2) with a similar molecular weight of 31 kDa were successfully isolated and purified from the 3-day deCSP-containing medium. Both P1 and P2 exhibited the highest activity of gelatin hydrolysis at pH 6 and 60 °C. The gelatin hydrolysates catalyzed by Paenibacillus TKU052 proteases were evaluated for biological activities, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, angiotensin-I converting enzyme (ACE) inhibition, and prebiotic activities. The gelatin hydrolysates expressed 31.76–43.95% DPPH radical scavenging activity and 31.58–36.84% ACE inhibitory activity, which was higher than those from gelatin. Gelatin hydrolysates also showed the growth-enhancing effect on Bifidobacterium bifidum BCRC 14615 with an increase to 135.70–147.81%. In short, Paenibacillus sp. TKU052 could be a potential strain to utilize crab shell wastes to produce proteases for bio-active peptides’ preparation. |
|---|
| 關鍵字 | angiotensin-I converting enzyme inhibitory activity;free radical scavenging activity;Paenibacillus;peptide;prebiotic;protease |
|---|
| 語言 | 英文 |
|---|
| ISSN | |
|---|
| 期刊性質 | 國外 |
|---|
| 收錄於 | SCI; |
|---|
| 產學合作 | |
|---|
| 通訊作者 | San-Lang Wang |
|---|
| 審稿制度 | 是 |
|---|
| 國別 | 瑞士 |
|---|
| 公開徵稿 | |
|---|
| 出版型式 | ,電子版 |
|---|
| 相關連結 | |
|---|
|
Google+ 推薦功能，讓全世界都能看到您的推薦！
