教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:Utilization of crab waste for cost-effective bioproduction of prodigiosin
學年109
學期1
出版(發表)日期2020/10/22
作品名稱Utilization of crab waste for cost-effective bioproduction of prodigiosin
作品名稱(其他語言)
著者Nguyen VB; Nguyen DM; Nguyen AD; Ngo VA; Ton TQ; Doan CT; Pham TP; Tran TPH; Wang SL
單位
出版者
著錄名稱、卷期、頁數Marine Drugs 18(11), 523
摘要This study aimed to establish the culture process for the cost-effective production of prodigiosin (PG) from demineralized crab shell powder (de-CSP), a fishery processing byproduct created via fermentation. Among the tested PG-producing strains, Serratia marcescens TNU02 was demonstrated to be the most active strain. Various ratios of protein/de-CSP were used as the sources of C/N for PG biosynthesis. The PG yield was significantly enhanced when the casein/de-CSP ratio was controlled in the range of 3/7 to 4/6. TNU02 produced PG with a high yield (5100 mg/L) in a 15 L bioreactor system containing 4.5 L of a newly-designed liquid medium containing 1.6% C/N source (protein/de-CSP ratio of 3/7), 0.02% (NH4)2SO4, 0.1% K2HPO4, and an initial pH of 6.15, at 27 °C for 8 h in dark conditions. The red pigment was purified from the culture broth and then quantified as being PG by specific Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) and UV spectra analysis. The purified PG demonstrated moderate antioxidant and effective inhibition against four cancerous cell lines. Notably, this study was the first to report on using crab wastes for PG bioproduction with high-level productivity (5100 mg/L) in a large scale (4.5 L per pilot) in a short period of fermentation time (8 h). The salt compositions, including (NH4)2SO4 and K2HPO4, were also a novel finding for the enhancement of PG yield by S. marcescens in this report.
關鍵字Serratia marcescens;prodigiosin;bioreactor system;crab shells;bioprocessing;antioxidants;anticancers
語言英文
ISSN1660-3397
期刊性質國外
收錄於SCI;
產學合作
通訊作者WANG San-Lang
審稿制度
國別瑞士
公開徵稿
出版型式,電子版
相關連結
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