教師資料查詢 | 類別: 期刊論文 | 教師: 陳俊成 LUKE CHEN (瀏覽個人網頁)

標題:ZnO Nanoparticles Induced Caspase-Dependent Apoptosis in Gingival Squamous Cell Carcinoma through Mitochondrial Dysfunction and p70S6K Signaling Pathway
學年108
學期2
出版(發表)日期2020/02/26
作品名稱ZnO Nanoparticles Induced Caspase-Dependent Apoptosis in Gingival Squamous Cell Carcinoma through Mitochondrial Dysfunction and p70S6K Signaling Pathway
作品名稱(其他語言)
著者Shih-Wei Wang; Chien-Hsing Lee; Ming-Shen Lin; Chih-Wen Chi; Yu-Jen Chen; Guo-Shou Wang; Kuang-Wen Liao; Li-Pin Chiu; Shu-Hui Wu; Dong-Ming Huang; Luke Chen; Yung-Shuen Shen
單位
出版者
著錄名稱、卷期、頁數International Journal of Molecular Science 21(5), 1612
摘要Zinc oxide nanoparticles (ZnO-NPs) are increasingly used in sunscreens, food additives,
pigments, rubber manufacture, and electronic materials. Several studies have shown that ZnO-NPs
inhibit cell growth and induce apoptosis by the production of oxidative stress in a variety of
human cancer cells. However, the anti-cancer property and molecular mechanism of ZnO-NPs in
human gingival squamous cell carcinoma (GSCC) are not fully understood. In this study, we found
that ZnO-NPs induced growth inhibition of GSCC (Ca9-22 and OECM-1 cells), but no damage in
human normal keratinocytes (HaCaT cells) and gingival fibroblasts (HGF-1 cells). ZnO-NPs caused
apoptotic cell death of GSCC in a concentration-dependent manner by the quantitative assessment of
oligonucleosomalDNAfragmentation. Flow cytometric analysis of cell cycle progression revealed that
sub-G1 phase accumulation was dramatically induced by ZnO-NPs. In addition, ZnO-NPs increased
the intracellular reactive oxygen species and specifically superoxide levels, and also decreased the
mitochondrial membrane potential. ZnO-NPs further activated apoptotic cell death via the caspase
cascades. Importantly, anti-oxidant and caspase inhibitor clearly prevented ZnO-NP-induced cell
Int. J. Mol. Sci. 2020, 21, 1612; doi:10.3390/ijms21051612 www.mdpi.com/journal/ijms
Int. J. Mol. Sci. 2020, 21, 1612 2 of 16
death, indicating the fact that superoxide-induced mitochondrial dysfunction is associated with the
ZnO-NP-mediated caspase-dependent apoptosis in human GSCC. Moreover, ZnO-NPs significantly
inhibited the phosphorylation of ribosomal protein S6 kinase (p70S6K kinase). In a corollary in vivo
study, our results demonstrated that ZnO-NPs possessed an anti-cancer e ect in a zebrafish xenograft
model. Collectively, these results suggest that ZnO-NPs induce apoptosis through the mitochondrial
oxidative damage and p70S6K signaling pathway in human GSCC. The present study may provide
an experimental basis for ZnO-NPs to be considered as a promising novel anti-tumor agent for the
treatment of gingival cancer.
關鍵字zinc oxide nanoparticles;gingival cancer;superoxide;p70S6K pathway
語言英文
ISSN1422-0067
期刊性質國外
收錄於SCI;
產學合作
通訊作者Shih-WeiWang
審稿制度
國別瑞士
公開徵稿
出版型式,電子版
相關連結
Google+ 推薦功能,讓全世界都能看到您的推薦!