教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:Phytophthora antagonism of endophytic bacteria isolated from roots of black pepper (Piper nigrum L.).
學年108
學期2
出版(發表)日期2020/02/17
作品名稱Phytophthora antagonism of endophytic bacteria isolated from roots of black pepper (Piper nigrum L.).
作品名稱(其他語言)
著者Ngo VA, Wang SL*, Nguyen VB, Doan CT, Tran TN, Tran DM, Tran TD, Nguyen AD
單位
出版者
著錄名稱、卷期、頁數Agronomy 10(2), 286
摘要Abstract: In this study, 90 root samples were collected from 30 black pepper farms in three provinces in the Central Highlands of Vietnam. A total of 352 endophytic bacteria were isolated and their morphology described. An in vitro assay on the ntifungal activity of these isolates was then conducted and 47 isolates were found to have antagonistic activity on Phytophthora fungi. The antifungal activity of the 47 isolates was evaluated in vivo by shoot assay. Among these 47 isolates, 6 were selected for further investigation. The six isolates were classified and
identified by sequencing the 16S RNA gene and phylogeny. The results showed that all six endophytic bacteria belong to the following species of Bacillus genus: B. siamensis, B. amyloliquefaciens, B. velezenis, and B. methylotrophiycus. Enzymatic activity related to the antifungal activity of the six potent isolates was determined; it showed that they possessed high chitinase and protease activities. These isolates were
applied for black pepper seedlings in greenhouse. The results showed three promising isolates: B. siamensis EB.CP6, B. velezensis EB.KN12, and B. methylotrophycus EB.KN13. Black pepper seedlings
treated with the promising bacteria had the lowest rate of root disease (8.45–11.21%) and lower fatal rate (11.11–15.55%) compared to the control group (24.81% and 24.44%). In addition, the three
promising isolates strongly affected the growth of the black pepper seedlings in greenhouse. The plant height, length of roots, and fresh biomass of the seedlings in the treated plots were higher than
those in the control plots. Thus, the endophytic bacterial isolates have the potential to act as biocontrol agent for the sustainable production of black pepper.
關鍵字endophytic bacteria;black pepper;Bacillus velezensis / siamensis/ methylotrophycus; antifungal activity;Phytophthora
語言英文
ISSN2073-4395
期刊性質國外
收錄於SCI;
產學合作
通訊作者Wang, San-Lang
審稿制度
國別瑞士
公開徵稿
出版型式,電子版
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