教師資料查詢 | 類別: 期刊論文 | 教師: 蔡旻燁 MIN-YEH TSAI (瀏覽個人網頁)

標題:Intrinsic coordination for revealing local structural changes in protein folding-unfolding
學年104
學期1
出版(發表)日期2015/12/23
作品名稱Intrinsic coordination for revealing local structural changes in protein folding-unfolding
作品名稱(其他語言)
著者Shiu, Ying-Jen; Hayashi, Michitoshi; Shih, Orion; Su, Charlene; Tsai, Min-Yeh; Yeh, Yi-Qi; Su, Chun-Jen; Huang, Yu-Shan; Lin, Sheng-Hsien; Jeng, U-Ser
單位
出版者
著錄名稱、卷期、頁數Physical Chemistry Chemical Physics, 18(4), 3179
摘要With a deformed object of a rigid rod inside, the local dislocations may be tracked relatively easily with respect to the internal rigid rod. We apply this concept on protein folding-unfolding to track the internal structural changes of an unfolded protein in solution. Proposed here is a protein internal coordination based on the major axis X of an ellipsoidal protein and the stable intrinsic transition dipole moment μ of the protein during unfolding. In this methodology, small-angle X-ray scattering (SAXS) is used to provide the protein global morphologies in the native and unfolded states. Furthermore, time-resolved fluorescence anisotropy (TRFA) provides the relative orientation between X and μ of Trp59 of the model protein cytochrome c. Hence observed in the protein unfolding with denaturants, acid, urea, or GuHCl, is the elongation of the native protein conformation along a reoriented protein major axis; accompanied are the different extents of relocations of the terminal α helices and loop structures of the protein in the corresponding unfolding.
關鍵字
語言英文
ISSN1463-9076
期刊性質國外
收錄於SCI;
產學合作
通訊作者
審稿制度
國別英國
公開徵稿
出版型式,電子版,紙本
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