教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:Bioprocessing shrimp shells to rat intestinal α- glucosidase inhibitor and its effect on reducing blood glucose in a mouse model
學年108
學期1
出版(發表)日期2019/10/01
作品名稱Bioprocessing shrimp shells to rat intestinal α- glucosidase inhibitor and its effect on reducing blood glucose in a mouse model
作品名稱(其他語言)
著者Nguyen VB; Nguyen TH; Nguyen AD; Le T; Kuo YH; Wang SL
單位
出版者
著錄名稱、卷期、頁數Research on Chemical Intermediates 45(10), p.4829-4846
摘要The α-Glucosidase inhibitors (aGIs) from natural resources have been recognized
as a safe and efficacious way to manage type 2 diabetes—an ongoing global health
problem. The objective of this study was to synthesize aGIs from demineralized
shrimp shell powder (deSSP), a low-cost fishery processing by-product, via microbial
fermentation. Its effect on reducing plasma glucose in an ICR mouse model was
then evaluated, and its other beneficial bioactivities investigated. Among the various
marine chitinous materials, fermented deSSP (fdeSSP) via Paenibacillus conversion
demonstrated potent inhibition against rat intestinal α-glucosidase that was
comparable to acarbose, a commercial anti-diabetic drug, with max inhibition and
IC50
values 87% and 120 μg/mL, and 89% and 110 μg/mL, respectively. After optimization
of the fermentation process, activity increased 1.52-fold (2500–3800 U/
mL). By comparing the HPLC fingerprints and bioactivity of fermented and unfermented
deSSP, it was confirmed that aGIs were produced as they were not present
in the deSSP medium prior to fermentation. In animal tests, at a dose of 100 mg/kg
bw, fdeSSP significantly reduced plasma glucose in ICR mice with no signs of diarrhea.
The fdeSSP also demonstrated acceptable antioxidant activity (
IC50 = 250 μg/
mL) and potential anti-NO activity (70.08 μg/mL). Though not toxic to normal cells,
fdeSSP reduced the viability of some cancer cells, including A549, Hep G2, MCF-7
and WIDR with inhibition values of 78%, 71%, 45% and 35%, respectively. The
results above suggest that fdeSSP may be a good candidate for development as a
health food or drug, due to its potential hypoglycemic and added value bioactivities.
關鍵字Shrimp shells;α-Glucosidase inhibitors;Anti-cancer;Diabetes;Process biochemistry
語言英文
ISSN0922-6168
期刊性質國外
收錄於SCI;
產學合作
通訊作者San-Lang Wang
審稿制度
國別美國
公開徵稿
出版型式,電子版
相關連結
Google+ 推薦功能,讓全世界都能看到您的推薦!