教師資料查詢 | 類別: 期刊論文 | 教師: 謝璦如 HSIEH, AI-RU (瀏覽個人網頁)

標題:A Novel Method for Identification and Quantification of Consistently Differentially Methylated Regions
學年102
學期2
出版(發表)日期2014/05/12
作品名稱A Novel Method for Identification and Quantification of Consistently Differentially Methylated Regions
作品名稱(其他語言)
著者Hsiao CL, Hsieh Ai-Ru, Lian IB, Lin YC, Wang HM, Cathy S. J. Fann
單位
出版者
著錄名稱、卷期、頁數PLoS One 9(5), p.e97513
摘要Advances in biotechnology have resulted in large-scale studies of DNA methylation. A differentially methylated region (DMR) is a genomic region with multiple adjacent CpG sites that exhibit different methylation statuses among multiple samples. Many so-called “supervised” methods have been established to identify DMRs between two or more comparison groups. Methods for the identification of DMRs without reference to phenotypic information are, however, less well studied. An alternative “unsupervised” approach was proposed, in which DMRs in studied samples were identified with consideration of nature dependence structure of methylation measurements between neighboring probes from tiling arrays. Through simulation study, we investigated effects of dependencies between neighboring probes on determining DMRs where a lot of spurious signals would be produced if the methylation data were analyzed independently of the probe. In contrast, our newly proposed method could successfully correct for this effect with a well-controlled false positive rate and a comparable sensitivity. By applying to two real datasets, we demonstrated that our method could provide a global picture of methylation variation in studied samples. R source codes to implement the proposed method were freely available at http://www.csjfann.ibms.sinica.edu.tw/eag/programlist/ICDMR/ICDMR.html.
關鍵字
語言中文
ISSN
期刊性質國內
收錄於
產學合作
通訊作者
審稿制度
國別中華民國
公開徵稿
出版型式,電子版
相關連結
Google+ 推薦功能,讓全世界都能看到您的推薦!