教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:Anti-α-glucosidase activity by a protease from Bacillus licheniformis
學年107
學期2
出版(發表)日期2019/02/15
作品名稱Anti-α-glucosidase activity by a protease from Bacillus licheniformis
作品名稱(其他語言)
著者Chien Thang Doan; Thi Ngoc Tran; Minh Trung Nguyen; Van Bon Nguyen; Anh Dzung Nguyen; San-Lang Wang
單位
出版者
著錄名稱、卷期、頁數Molecules 24(4), 691(13 pages)
摘要Anti-α-glucosidase (AAG) compounds have received great attention due to their potential
use in treating diabetes. In this study, Bacillus licheniformis TKU004, an isolated bacterial strain
from Taiwanese soil, produced AAG activity in the culture supernatant when squid pens were
used as the sole carbon/nitrogen (C/N) source. The protein TKU004P, which was isolated from
B. licheniformis TKU004, showed stronger AAG activity than acarbose, a commercial anti-diabetic
drug (IC50 = 0.1 mg/mL and 2.02 mg/mL, respectively). The molecular weight of TKU004P,
determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), was 29 kDa.
High-performance liquid chromatography (HPLC) analysis showed that TKU004P may be a protease
that demonstrates AAG activity by degrading yeast α-glucosidase. Among the four chitinous sources
of C/N, TKU004P produced the highest AAG activity in the culture supernatant when shrimp head
powder was used as the sole source (470.66 U/mL). For comparison, 16 proteases, were investigated
for AAG activity but TKU004P produced the highest levels. Overall, the findings suggest that
TKU004P could have applications in the biochemical and medicinal fields thanks to its ability to
control the activity of α-glucosidase.
關鍵字anti-α-glucosidase;protease;diabetes;microbial conversion;Bacillus licheniformis
語言英文
ISSN1420-3049
期刊性質國外
收錄於SCI;
產學合作
通訊作者WANG San-Lang
審稿制度
國別瑞士
公開徵稿
出版型式,電子版
相關連結
Google+ 推薦功能,讓全世界都能看到您的推薦!