教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:Reclamation of marine chitinous materials for chitinase and protease production via microbial conversion by Paenibacillus macerans
學年107
學期1
出版(發表)日期2018/11/02
作品名稱Reclamation of marine chitinous materials for chitinase and protease production via microbial conversion by Paenibacillus macerans
作品名稱(其他語言)
著者Doan CT; Tran TN, Nguyen VB; Nguyen AD; Wang SL
單位
出版者
著錄名稱、卷期、頁數Marine Drugs, 16(11), p.429
摘要: Chitinous materials from marine byproducts elicit great interest among biotechnologists for
their potential biomedical or agricultural applications. In this study, four kinds of marine chitinous
materials (squid pens, shrimp heads, demineralized shrimp shells, and demineralized crab shells)
were used to screen the best source for producing chitosanase by Paenibacillus macerans TKU029.
Among them, the chitosanase activity was found to be highest in the culture using the medium
containing squid pens as the sole carbon/nitrogen (C/N) source. A chitosanase which showed
molecular weights at 63 kDa was isolated from P. macerans cultured on a squid pens medium.
The purified TKU029 chitosanase exhibited optimum activity at 60 ◦C and pH 7, and was stable at
temperatures under 50 ◦C and pH 3-8. An analysis by MALDI-TOF MS revealed that the chitosan
oligosaccharides (COS) obtained from the hydrolysis of water-soluble chitosan by TKU029 crude
enzyme showed various degrees of polymerization (DP), varying from 3–6. The obtained COS
enhanced the growth of four lactic acid bacteria strains but exhibited no effect on the growth of E. coli.
By specialized growth enhancing effects, the COS produced from hydrolyzing water soluble chitosan
with TKU029 chitinolytic enzymes could have potential for use in medicine or nutraceuticals.
關鍵字chitin; chitosan; protease; chitinase; chitosan oligomers
語言英文
ISSN1660-3397
期刊性質國外
收錄於SCI;
產學合作
通訊作者San-Lang Wang
審稿制度
國別瑞士
公開徵稿
出版型式,電子版
相關連結
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