教師資料查詢 | 類別: 期刊論文 | 教師: 董崇民 Don, Trong-ming (瀏覽個人網頁)

標題:Temperature/pH/Enzyme Triple-Responsive Cationic Protein/PAA‑b‑PNIPAAm Nanogels for Controlled Anticancer Drug and Photosensitizer Delivery against Multidrug Resistant Breast Cancer Cells
學年106
學期1
出版(發表)日期2017/12/04
作品名稱Temperature/pH/Enzyme Triple-Responsive Cationic Protein/PAA‑b‑PNIPAAm Nanogels for Controlled Anticancer Drug and Photosensitizer Delivery against Multidrug Resistant Breast Cancer Cells
作品名稱(其他語言)
著者Trong-Ming Don; Kun-Ying Lu; Li-Jie Lin; Chun-Hua Hsu; Jui-Yu Wu; Fwu-Long Mi
單位
出版者
著錄名稱、卷期、頁數Molecular Pharmaceutics 14(12), p.4648−4660
摘要The tumor microenvironments are often acidic and overexpress specific enzymes. In this work, we synthesized a poly(AA-b-NIPAAm) copolymer (PAA-b-PNIPAAm) using a reversible addition-fragmentation chain transfer (RAFT) polymerization method. PAA-b-PNIPAAm and a cationic protein (protamine) were self-assembled into nanogels, which effectively reduced the cytotoxicity of protamine. The protamine/PAA-b-PNIPAAm nanogels were responsive to the stimuli including temperature, pH, and enzyme due to disaggregation of PAA-b-PNIPAAm, change in random coil/α-helix conformation of protamine, and enzymatic hydrolysis of the protein. Changing the pH from 7.4 to a lowered pHe (6.5-5.0) resulted in an increase in mean particle size and smartly converted surface charge from negative to positive. The cationic nanogels easily passed through the cell membrane and enhanced intracellular localization and accumulation of doxorubicin-loaded nanogels in multidrug resistant MCF-7/ADR breast cancer cells. Cold shock treatment triggered rapid intracellular release of doxorubicin against P-glycoprotein (Pgp)-mediated drug efflux, showing significantly improved anticancer efficacy as compared with free DOX. Furthermore, the nanogels were able to carry a rose bengal photosensitizer and caused significant damage to the multidrug resistant cancer cells under irradiation. The cationic nanogels with stimuli-responsive properties show promise as drug carrier for chemotherapy and photodynamic therapy against cancers.
關鍵字N-isopropylacrylamide;enzymatic digestion;nanogels;pH-responsive;protamine;thermoresponsive
語言英文(美國)
ISSN
期刊性質國外
收錄於SCI;
產學合作
通訊作者Fwu-Long Mi
審稿制度
國別美國
公開徵稿
出版型式,電子版
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