教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:Conversion of squid pens to chitosanases and proteases via Paenibacillus sp TKU042
學年106
學期2
出版(發表)日期2018/03/06
作品名稱Conversion of squid pens to chitosanases and proteases via Paenibacillus sp TKU042
作品名稱(其他語言)
著者Doan CT; Tran TN; Nguyen VB; Nguyen AD; Wang SL
單位
出版者
著錄名稱、卷期、頁數Marine Drugs 16(83), pp.1-12
摘要Chitosanases and proteases have received much attention due to their wide range of applications. Four kinds of chitinous materials, squid pens, shrimp heads, demineralized shrimp shells and demineralized crab shells, were used as the sole carbon and nitrogen (C/N) source to produce chitosanases, proteases and α-glucosidase inhibitors (αGI) by four different strains of Paenibacillus. Chitosanase productivity was highest in the culture supernatants using squid pens as the sole C/N source. The maximum chitosanase activity of fermented squid pens (0.759 U/mL) was compared to that of fermented shrimp heads (0.397 U/mL), demineralized shrimp shells (0.201 U/mL) and demineralized crab shells (0.216 U/mL). A squid pen concentration of 0.5% was suitable for chitosanase, protease and αGI production via Paenibacillus sp. TKU042. Multi-purification, including ethanol precipitation and column chromatography of Macro-Prep High S as well as Macro-Prep DEAE (diethylaminoethyl), led to the isolation of Paenibacillus sp. TKU042 chitosanase and protease with molecular weights of 70 and 35 kDa, respectively. For comparison, 16 chitinolytic bacteria, including strains of Paenibacillus, were investigated for the production of chitinase, exochitinase, chitosanase, protease and αGI using two kinds of chitinous sources.
關鍵字Paenibacillus;chitin;chitosan;chitosanase;protease;squid pens;α-glucosidase inhibitors
語言英文
ISSN1660-3397
期刊性質國外
收錄於SCI;
產學合作
通訊作者WANG San-Lang
審稿制度
國別中華民國
公開徵稿
出版型式,電子版
相關連結
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