教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:Utilization of Fishery Processing By-Product Squid Pens for -Glucosidase Inhibitors Production by Paenibacillus sp.
學年106
學期1
出版(發表)日期2017/08/30
作品名稱Utilization of Fishery Processing By-Product Squid Pens for -Glucosidase Inhibitors Production by Paenibacillus sp.
作品名稱(其他語言)
著者Nguyen VB; Nguyen AD; Wang SL
單位
出版者
著錄名稱、卷期、頁數Marine Drugs, 15(9), p.274(11 pages)
摘要The supernatants (the solution part received after centrifugation) of squid pens fermented
by four species of Paenibacillus showed potent inhibitory activity against α-glucosidases derived
from yeast (79–98%) and rats (76–83%). The inhibition of acarbose—a commercial antidiabetic
drug, used against yeast and rat α-glucosidases—was tested for comparison; it showed inhibitory
activity of 64% and 88%, respectively. Other chitinolytic or proteolytic enzyme-producing bacterial
strains were also used to ferment squid pens, but no inhibition activity was detected from the
supernatants. Paenibacillus sp. TKU042, the most active α-glucosidase inhibitor (aGI)-producing
strain, was selected to determine the optimal cultivation parameters. This bacterium achieved the
highest aGI productivity (527 µg/mL) when 1% squid pens were used as the sole carbon/nitrogen
source with a medium volume of 130 mL (initial pH 6.85) in a 250 mL flask (48% of air head space), at
30 ◦C for 3–4 d. The aGI productivity increased 3.1-fold after optimization of the culture conditions.
Some valuable characteristics of Paenibacillus aGIs were also studied, including pH and thermal
stability and specific inhibitory activity. These microbial aGIs showed efficient inhibition against
α-glucosidases from rat, yeast, and bacteria, but weak inhibition against rice α-glucosidase with IC50
values of 362, 252, 189, and 773 µg/mL, respectively. In particular, these aGIs showed highly stable
activity over a large pH (2–13) and temperature range (40–100 ◦C). Various techniques, including:
Diaoin, Octadecylsilane opened columns, and preparative HPLC coupled with testing bioactivity
resulted in isolating a main active compound; this major inhibitor was identified as homogentisic acid
(HGA). Notably, HGA was confirmed as a new inhibitor, a non-sugar-based aGI, and as possessing
stronger activity than acarbose with IC50, and maximum inhibition values of 220 µg/mL, 95%, and
1510 µg/mL, 65%, respectively. These results suggest that squid pens, an abundant and low-cost
fishery processing by-product, constitute a viable source for the production of antidiabetic materials
via fermentation by strains of Paenibacillus. This fermented product shows promising applications in
diabetes or diabetes related to obesity treatment due to their stability, potent bioactivity, and efficient
inhibition against mammalian enzymes.
關鍵字squid pens;α-glucosidase inhibitor;Paenibacillus species;diabetes;obesity;microbial conversion;fishery processing;homogentisic acid
語言英文
ISSN1660-3397
期刊性質國外
收錄於SCI;
產學合作
通訊作者WANG San-Lang
審稿制度
國別瑞士
公開徵稿
出版型式,電子版
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