教師資料查詢 | 類別: 期刊論文 | 教師: 莊程豪 CHENG-HAO CHUANG (瀏覽個人網頁)

標題:Detecting trypsin at liquid crystal/aqueous interface by using surface-immobilized bovine serum albumin
學年104
學期2
出版(發表)日期2016/04/15
作品名稱Detecting trypsin at liquid crystal/aqueous interface by using surface-immobilized bovine serum albumin
作品名稱(其他語言)
著者Cheng-Hao Chuang; Yi-Cheng Lin; You-Shuan Chen; Yu-Xun Chen; Chieh-Ming Chen; Hung Wei Shiu; Lo-Yueh Chang; Chia-Hao Chen; Chih-Hsin Chen
單位
出版者
著錄名稱、卷期、頁數Biosensors and Bioelectronics 78, p.213–220
摘要We report a new mechanism for liquid crystal (LC)-based sensor system for trypsin detection. In this system, bovine serum albumin (BSA) was immobilized on gold grids as the enzymatic substrate. When the BSA-modified grid was filled with LC and immersed in the solution containing trypsin, the peptide bonds of BSA were hydrolyzed and peptide fragments were desorbed from the surface of gold grid, which disrupted the orientation of LC at the vicinity and resulted in a dark-to-bright transition of optical image of LCs. By using this mechanism, the limit of detection (LOD) of trypsin is 10 ng/mL, and it does not respond to thrombin and pepsin. Besides, the cleavage behavior on gold surfaces was directly visualized by the scanning photoelectron microscopy (SPEM), in particular for the chemical composition identification and element-resolved image. The loss of BSA fragments and the enhancement of Au photoelectron signal after trypsin cleavage were corresponding to the proposed mechanism of the LC-based sensor system. Because the signals reported by LC can be simply interpreted through the human naked-eye, it provides a simple method for fast-screening trypsin activity in aqueous solution.
關鍵字Liquid crystal-based biosensor;Bovine serum albumin;Trypsin;Scanning photoelectron microscopy
語言英文
ISSN0956-5663
期刊性質國外
收錄於SCI;
產學合作
通訊作者Chih-Hsin Chen
審稿制度
國別荷蘭
公開徵稿
出版型式,電子版,紙本
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