教師資料查詢 | 類別: 期刊論文 | 教師: 王伯昌 WANG, BO-CHENG (瀏覽個人網頁)

標題:Evaluation of the teratogenic effects of three traditional Chinese medicines, Si Jun Zi Tang, Liu Jun Zi Tang and Shenling Baizhu San, during zebrafish pronephros development
學年104
學期1
出版(發表)日期2015/12/11
作品名稱Evaluation of the teratogenic effects of three traditional Chinese medicines, Si Jun Zi Tang, Liu Jun Zi Tang and Shenling Baizhu San, during zebrafish pronephros development
作品名稱(其他語言)
著者Ding, Yu-Ju; Wang, Bo-Cheng; Wen, Chi-Chung; Sun, Chiao-Yin; Lee, Hsun-Hua; Lee, Fei-Peng; Yang, Ling-Ling; Chen, Yau-Hung
單位
出版者
著錄名稱、卷期、頁數Journal of Toxicologic Pathology 28(3), pp.141-149
摘要The aim of this study was to evaluate the teratogenic effects of three common Chinese medical prescriptions, Si Jun Zi Tang (SJZT), Liu Jun Zi Tang (LJZT) and Shenling Baizhu San (SLBS), during zebrafish pronephros development. We used the transgenic zebrafish line Tg(wt1b:EGFP) to assess the teratogenic effects using 12 different protocols, which comprised combinations of 4 doses (0, 25, 250, 1,250 ng/mL) and 3 exposure methods [methods I, 12–36 hours post fertilization (hpf), II, 24–48 hpf, and III, 24–36 hpf]. As a result, few defects in the kidneys were observed in the embryos exposed to 25 ng/mL of each medical prescription. The percentage of kidney malformation phenotypes increased as the exposure concentrations increased (25 ng/mL, 0–10%; 250 ng/mL, 0–60%; 1,250 ng/mL, 80–100%). Immunohistochemistry for α6F, which is a basolateral and renal tubular differentiation marker, revealed no obvious defective phenotypes in either SJZT- or LJZT-treated embryos, indicating that these Chinese medical prescriptions had minimal adverse effects on the pronephric duct. However, SLBS-treated embryos displayed a defective phenotype in the pronephric duct. According to these findings, we suggest (1) that the Chinese medical prescriptions induced kidney malformation phenotypes that are dose dependent and (2) that the embryonic zebrafish kidney was more sensitive to SLBS than SJZT and LJZT.
關鍵字Chinese medical prescriptions;kidney;nephrotoxicity;zebrafish
語言英文
ISSN1881-915X ; 0914-9198
期刊性質國外
收錄於SCI;
產學合作
通訊作者Yang, Ling-Ling; Chen, Yau-Hung
審稿制度
國別日本
公開徵稿
出版型式,電子版,紙本
相關連結
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