教師資料查詢 | 類別: 期刊論文 | 教師: 陳志欣 CHEN, CHIH-HSIN (瀏覽個人網頁)

標題:Liquid crystal-based immunoassay for detecting human serum albumin
學年102
學期2
出版(發表)日期2014/03/27
作品名稱Liquid crystal-based immunoassay for detecting human serum albumin
作品名稱(其他語言)
著者Chen, Chih-hsin; Ho, Wen-hsun
單位淡江大學化學學系
出版者Dordrecht: Springer Netherlands
著錄名稱、卷期、頁數Research on Chemical Intermediates 40(6), pp.2229-2236
摘要Human serum albumin (HSA) is the most abundant protein in human blood plasma. It is commonly used as a biomarker in urine samples to identify the chronic kidney disease caused by high blood pressure or diabetes. In our research, a thin layer of liquid crystals (LCs) is used as a readout system for developing an immunoassay that reports the presence of HSA in the aqueous solution with optical signals. The detection principle of this assay is based on the variation of surface density of protein upon the specific binding of HSA on an anti-HSA immobilized surface, which leads a dark-to-bright transition of LC images under cross-polarizers. Our results show that the LC-based immunoassay can detect HSA at a concentration of 50 μg/mL. By using the slide with immobilized anti-HSA in array format, the concentration level of HSA can be simply determined by the number of LC spot shown on the slide.
關鍵字Liquid crystal;Immunoassay;Human serum albumin;Microalbuminuria
語言英文
ISSN1568-5675
期刊性質國外
收錄於SCI;
產學合作
通訊作者Chen, Chih-Hsin
審稿制度
國別荷蘭
公開徵稿
出版型式,電子版,紙本
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