教師資料查詢 | 類別: 期刊論文 | 教師: 董崇民 Don, Trong-ming (瀏覽個人網頁)

標題:Enzymatic extruded starch as a carbon source for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Haloferax mediterranei
學年95
學期1
出版(發表)日期2006/11/01
作品名稱Enzymatic extruded starch as a carbon source for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Haloferax mediterranei
作品名稱(其他語言)
著者Chen, C. Will; 董崇民; Don, Trong-ming; Yen, Hsiao-feng
單位淡江大學化學工程與材料工程學系
出版者Elsevier
著錄名稱、卷期、頁數Process Biochemistry 41(11), pp.2289-2296
摘要The extremely halophilic Archaeon Haloferax mediterranei can accumulate polyhydroxyalkanoate (PHA) on a medium containing high salt concentration. The available carbon source for the cell growth and PHA production was the native cornstarch being treated by an enzymatic reactive extrusion process. The extrusion process was processed at a 25 mm single-screw extruder with α-amylase quantities at 1–5 g/100 g wet weight of cornstarch, screw speed at 39–78 rpm and temperatures controlled at 55 °C for zone 1, 65 °C for zone 2, 85 °C for zone 3 and 90 °C for the die. By using an extruded starch/yeast extract ratio (ES/YE ratio) of 1/1.7 g/g in the feeding medium to maintain starch and organic nitrogen concentrations during the pH-stat fed-batch fermentation, the PHA content could reach 50.8% (w/w) of dry cell. From the nuclear magnetic resonance (1H NMR) spectrum, the PHA was confirmed to be a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) copolymer with a composition of 10.4 mol% of HV unit. The glass transition temperature of this copolymer was −1.2 °C as measured by a differential scanning calorimeter. On the same thermogram, two melting peaks were found at 129.1 and 144.0 °C, respectively; which were much lower than those of the polyhydroxybutyrate (PHB) homopolymer.
關鍵字Haloferax mediterranei;Enzymatic extrusion;Poly(3-hydroxybutyrate-co-3-hydroxyvalerate);Starch;Fed-batch culture;pH-stat
語言英文
ISSN1359-5113
期刊性質國內
收錄於
產學合作
通訊作者
審稿制度
國別中華民國
公開徵稿
出版型式,電子版
相關連結
Google+ 推薦功能,讓全世界都能看到您的推薦!