Immobilization of l-lysine on dense and porous poly(vinylidene fluoride) surfaces for neuron culture
學年 97
學期 1
出版（發表）日期 2008-12-01
作品名稱 Immobilization of l-lysine on dense and porous poly(vinylidene fluoride) surfaces for neuron culture
作品名稱（其他語言）
著者 Young, Tai-horng; Lu, Jui-nan; Lin, Dar-jong; Chang, Chi-lin; Chang, Hsu-hsien; Cheng, Liao-ping
單位 淡江大學化學工程與材料工程學系
出版者 Amsterdam: Elsevier BV
著錄名稱、卷期、頁數 Desalination 234(1-3), pp. 134-143
摘要 Microporous poly(vinylidene fluoride) (PVDF) membranes with either dense or porous surface were prepared by isothermal immersion-precipitation of a casting solution in coagulation baths of different strengths. Onto the membrane surface, an amino acid (l-lysine) was immobilized by a dual-step chemical process. First, the membrane was grafted with poly(acrylic acid) (PAA) by means of plasma-induced free radical polymerization. Then, l-lysine was covalently bonded to the as-grafted PAA chains with the aid of a water-soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC). The highest attainable graft yield of PAA on PVDF membrane reached up to 0.64 mg/cm2. For immobilization of l-lysine on the membrane, the yields were found to depend on factors, such as concentration of EDC, activation time, and pH value. The maximal attainable immobilization yield was 0.65 μg/cm2. Furthermore, pheochromocytoma (PC12) cells were cultured on l-lysine/PAA/PVDF membranes. It was found that both the amount of l-lysine on the membrane and the surface structure had a marked influence on the cell activity. Thus, the present results could be useful for the development of strategies to promote the re-growth and regeneration of tissue in the nervous system.
關鍵字 Poly(vinylidene fluoride); Porous; Membrane; Immobilization; l-lysine
語言 en
ISSN 0011-9164
期刊性質 國外
收錄於 SCI EI
產學合作
通訊作者
審稿制度
國別 NLD
公開徵稿
出版型式 紙本
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