標題:Production and purification of a fungal chitosanase and chitooligomers from Penicillium janthinellum D4 and discovery of the enzyme activators |
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學年 | 103 |
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學期 | 1 |
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出版(發表)日期 | 2014/08/08 |
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作品名稱 | Production and purification of a fungal chitosanase and chitooligomers from Penicillium janthinellum D4 and discovery of the enzyme activators |
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作品名稱(其他語言) | |
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著者 | Anh Dzung Nguyen; Huang, Chun-Ching; Liang, Tzu-Wen; Van Bon Nguyen; Pan, Po-Shen; Wang, San-Lang |
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單位 | 淡江大學化學學系 |
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出版者 | Kidlington: Pergamon Press |
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著錄名稱、卷期、頁數 | Carbohydrate Polymers 108, pp.331–337 |
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摘要 | Chitosanases have received much attention because of their wide range of applications. Although most fungal chitosanases use sugar as their major carbon source, in the present work, a chitosanase was induced from a squid pen powder (SPP)-containing Penicillium janthinellum D4 medium and purified by ammonium sulphate precipitation and combined column chromatography. The purified D4 chitosanase exhibited optimum activity at pH 7–9, 60 °C and was stable at pH 7–11, 25–50 °C. The D4 chitosanase that was used for chitooligomers preparation was studied. The enzyme products revealed various chitooligomers with different degrees of polymerisation (DP) from 3 to 9, as determined by a MALDI-TOF mass spectrometer, confirming the endo-type nature of the D4 chitosanase. D4 chitosanase activity was significantly inhibited by Cu2+, Mn2+, and EDTA. However, Fe2+ activated or inhibited D4 chitosanases at different concentrations. The D4 chitosanase was also activated by some small synthetic boron-containing molecules with boronate ester side chains. |
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關鍵字 | Chitosanase; Squid pen; Penicillium janthinellum; Chitooligomers; Chitosanase activator; Boron |
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語言 | 英文 |
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ISSN | 0144-8617 |
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期刊性質 | 國外 |
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收錄於 | SCI; |
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產學合作 | |
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通訊作者 | Wang, San-Lang |
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審稿制度 | 是 |
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國別 | 英國 |
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公開徵稿 | |
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出版型式 | ,紙本 |
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相關連結 | |
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