教師資料查詢 | 類別: 期刊論文 | 教師: 梁慈雯 TZU-WEN LIANG (瀏覽個人網頁)

標題:Tyrosinase inhibitory activity of supernatant and semi-purified extracts from squid pen fermented with Burkholderia cepacia TKU025
學年102
學期2
出版(發表)日期2014/06/01
作品名稱Tyrosinase inhibitory activity of supernatant and semi-purified extracts from squid pen fermented with Burkholderia cepacia TKU025
作品名稱(其他語言)
著者Liang, Tzu-Wen; Lee, Yun-Chen; Wang, San-Lang
單位淡江大學化學學系
出版者Dordrecht: Springer Netherlands
著錄名稱、卷期、頁數Research on Chemical Intermediates
摘要Reports of tyrosinase inhibitors from microorganisms are rare. The tyrosinase inhibitory activity was found from the culture supernatant of Burkholderia cepacia TKU025 with 1 % squid pen as the sole carbon/nitrogen source. The fourth-day supernatant showed the strongest tyrosinase inhibitory activity (500 U/ml) and the highest total phenolic content (789 μg/ml GAE). The supernatant was further purified by liquid–liquid partition, and it was found that the methanol extract exhibited the lowest IC50 value of 2 μg/ml in the tyrosinase inhibitory activity assay and was compared with the positive control kojic acid and vitamin C. Significant associations between the tyrosinase inhibitory potency and the total phenolic content were found for the supernatant, extract, and fractions. The tyrosinase inhibitory activity persisted even when it was treated with acidic or alkaline conditions of pH 2 or 12. The tyrosinase inhibitory activity remained at 100 %, even after treatment at 100 °C for 60 min. The tyrosinase inhibitor also showed antimicrobial activity against Escherichia coli, Bacillus cereus, Aspergillus fumigatus, and Fusarium oxysporum.
關鍵字Tyrosinase inhibitor; Phenolic content; Squid pen; Antibacterial; Antifungal; Burkholderia cepacia
語言英文
ISSN0922-6168;1568-5675
期刊性質國外
收錄於
產學合作
通訊作者Wang, San-Lang
審稿制度
國別荷蘭
公開徵稿
出版型式紙本;電子版
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