教師資料查詢 | 類別: 會議論文 | 教師: 陳志欣 CHEN, CHIH-HSIN (瀏覽個人網頁)

標題:Liquid Crystal-based Immunoassay for Detecting Human Serum Albumin
學年102
學期1
發表日期2013/11/18
作品名稱Liquid Crystal-based Immunoassay for Detecting Human Serum Albumin
作品名稱(其他語言)
著者陳志欣; 何文薰
作品所屬單位淡江大學化學學系
出版者Springer
會議名稱The first OPU–TKU International Symposium on Frontier Chemistry and Materials for the 21st Century
會議地點Osaka, Japan
摘要Human serum albumin (HSA) is the most abundant protein in human blood plasma. It is commonly used as a biomarker in urine samples to identify the chronic kidney disease (CKD) caused by high blood pressure or diabetes. In our research, a thin layer of liquid crystals (LCs) is used as a readout system for developing an immunoassay that reports the presence of HSA in the aqueous solution with optical signals. The detection principle of this assay is based on the variation of surface density of protein upon the specific binding of HSA on anti-HSA immobilized surface, which leads a dark-to-bright transition of LC images under cross polarizers. Our results show that the LC-based immunoassay can detect HSA at concentration of 50 ug/mL. By using the slide with immobilized anti-HSA in array format, the concentration level of HSA can be simply determined by the number of LC spot shown on the slide.
關鍵字Liquid Crystal-based Immunoassay
語言英文
收錄於
會議性質國際
校內研討會地點
研討會時間20131118~20131119
通訊作者陳志欣
國別日本
公開徵稿Y
出版型式
出處The first OPU–TKU International Symposium on Frontier Chemistry and Materials for the 21st Century
相關連結
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