教師資料查詢 | 類別: 期刊論文 | 教師: 林諭男 I-nan Lin (瀏覽個人網頁)

標題:An amperometric urea biso sensor based on covalent immobilization of ureaseon N2 incorporated diamond nanowire electrode
學年102
學期1
出版(發表)日期2014/01/01
作品名稱An amperometric urea biso sensor based on covalent immobilization of ureaseon N2 incorporated diamond nanowire electrode
作品名稱(其他語言)
著者Jayakumar Shalini; Kamatchi Jothiramalingam Sankaran; Lee, Chi-Young; Tai, Nyan-Hwa; Lin, I-Nan
單位淡江大學物理學系
出版者Amsterdam: Elsevier BV
著錄名稱、卷期、頁數Biosensors and Bioelectronics 56, pp.64–70
摘要N2 incorporated diamond nanowire (N-DNW) film electrochemical biosensor has utilized for the quantitative determination of urea in aqueous solution and urine sample. N-DNW electrode is wet-chemically cleaned (oxidation) by boiling in a mixture of H2SO4 and HNO3 (3:1) at 200 °C for 2 h to remove graphite. Urease (Urs) and glutamate dehydrogenase (GLDH) are covalently attached to the oxidized N-DNW electrode by activating the COOH group of N-DNW using ethyl(dimethylaminopropyl)carbodiimide as the coupling agent and N-hydroxysuccinimide as activator. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy data reveal that carboxylic and hydroxyl functionalized nature of N-DNW electrodes Urs–GLDH immobilized N-DNW (Urs–GLDH/N-DNW) has been successfully utilized in urea biosensor which exhibits good performance in sensitivity (6.18 μA/mg dL/cm2), stability (~1 month), reproducibility, lower detection limit (3.87 mg/dL) and fast response time (>10 s). Urs–GLDH/N-DNW also exhibits electrochemical response when tested for different concentration of human urine in buffer solution (from 1:9 to 4:6). In addition, Urs–GLDH/N-DNW bioelectrode retains 80% of its initial enzyme activity for <1 month, when stored at 4–6 °C in a refrigerator.
關鍵字N2 incorporated diamond nanowire; Urs–GLDH/N-DNW;
Urea; Cyclic voltammetry; Electrochemical impedance spectrsocopy; Amperometry
語言英文(美國)
ISSN0956-5663;1873-4235
期刊性質國外
收錄於
產學合作
通訊作者Lee, Chi-Young
審稿制度
國別荷蘭
公開徵稿
出版型式紙本;電子版
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