教師資料查詢 | 類別: 期刊論文 | 教師: 陳曜鴻 CHEN, YAU-HUNG (瀏覽個人網頁)

標題:Outer membrane protein OmpF involved in the transportation of polypyridyl ruthenium complexes into Escherichia coli
學年98
學期2
出版(發表)日期2010/05/01
作品名稱Outer membrane protein OmpF involved in the transportation of polypyridyl ruthenium complexes into Escherichia coli
作品名稱(其他語言)
著者Ho, Ming-Yi; Chiou, Min-Lun; Chang, Ruei-Chi; Chen, Yau-Hung; Cheng, Chien-Chung
單位淡江大學化學學系
出版者Philadelphia: Elsevier Inc.
著錄名稱、卷期、頁數Journal of Inorganic Biochemistry 104(5), pp.614-617
摘要The discovery that OmpF was related to the transportation of ruthenium complexes through cell membrane was achieved with proteomics technologies. An integral ruthenium complex exists inside the cell as identified by matrix-assisted laser desorption ionization (MALDI) mass spectrometry. An inhibition assay with Escherichia coli was used to demonstrate the relationship between the transportation of the polypyridyl ruthenium complexes and the presence of OmpF (outer membrane protein F). For instance, the amount of [Ru(tpy)(bpy)Cl]+ (tpy: teripyridine; bpy: bipyridine) that entered the cells was determined by inductively coupled plasma optical emission spectroscopy (ICP-OES) of cell extracts and was measured to be approximately 0.55 μM. In the presence of 10% sucrose solution which is known to reduce the OmpF concentration, the ruthenium complex concentration was reduced to approximately 0.28 μM, which is a 50% reduction. These data suggest that OmpF plays a key role in the transportation of positively charged polypyridyl chlororuthenium complexes into E. coli.
關鍵字Ruthenium; Transportation; OmpF; MALDI; Metalloproteomics
語言英文(美國)
ISSN0162-0134;1873-3344
期刊性質國外
收錄於SCI
產學合作
通訊作者Cheng, Chien-Chung
審稿制度
國別美國
公開徵稿
出版型式紙本;電子版
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