教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:Conversion and degradation of shellfish wastes by Serratia sp. TKU016 fermentation for the production of enzymes and bioactive materials
學年98
學期1
出版(發表)日期2009/12/01
作品名稱Conversion and degradation of shellfish wastes by Serratia sp. TKU016 fermentation for the production of enzymes and bioactive materials
作品名稱(其他語言)
著者Wang, San-Lang; Chang, Tao-Jen; Liang, Tzu-Wen
單位淡江大學生命科學研究所
出版者Dordrecht: Springer Netherlands
著錄名稱、卷期、頁數Biodegradation 21(3), pp.321-333
摘要A chitosanase and a protease were purified from the culture supernatant of Serratia sp. TKU016 with shrimp shell as the sole carbon/nitrogen source. The molecular masses of the chitosanase and protease determined by SDS–PAGE were approximately 65 and 53 kDa, respectively. The chitosanase was inhibited completely by Mn2+, but the protease was enhanced by all of tested divalent metals. The optimum pH, optimum temperature, pH stability, and thermal stability of the chitosanase and protease were (pH 7, 50°C, pH 6–7, <50°C) and (pH 8–10, 40°C, pH 5–10, <50°C), respectively. SDS (2 mM) had stimulatory effect on TKU016 protease activity. The result demonstrates that TKU016 protease is SDS-resistant protease and probably has a rigid structure. Besides, TKU016 culture supernatant (2% SPP) incubated for 2 days has the highest antioxidant activity, the DPPH scavenging ability was about 76%. With this method, we have shown that shrimp shell wastes can be utilized and it’s effective in the production of enzymes, antioxidants, peptide and reducing sugar, facilitating its potential use in biological applications and functional foods.
關鍵字Antioxidant activity;Chitosanase;Protease;Serratia sp.;Shrimp shell wastes
語言英文
ISSN0923-9820
期刊性質國外
收錄於SCI;EI
產學合作
通訊作者Wang, San-Lang; Liang, Tzu-Wen
審稿制度
國別荷蘭
公開徵稿
出版型式紙本
相關連結
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