教師資料查詢 | 類別: 期刊論文 | 教師: 陳志欣 CHEN, CHIH-HSIN (瀏覽個人網頁)

標題:A Liquid Crystal Biosensor for Detecting Organophosphates through the Localized pH Changes Induced by Their Hydrolytic Products
學年101
學期2
出版(發表)日期2013/05/01
作品名稱A Liquid Crystal Biosensor for Detecting Organophosphates through the Localized pH Changes Induced by Their Hydrolytic Products
作品名稱(其他語言)
著者Chen, Chih-hsin; Yang, Kun-lin
單位淡江大學化學學系
出版者Lausanne: Elsevier S.A.
著錄名稱、卷期、頁數Sensors and Actuators B: Chemical 181, pp.368-374
摘要We report a biosensor for detecting organophosphates (OPs) by using liquid crystal (LC). The mechanism of the biosensor is based on detection of minute pH changes during the enzymatic hydrolysis of OPs. To hydrolyze OPs, paraoxonase 1 (PON1) is immobilized on a copper grid which also hosts pH-sensitive LC, 4-cyano-4′-pentylbiphenyl (5CB) doped with 0.3% of 4′-pentyl-biphenyl-4-carboxylic acid (PBA). Proximity of enzyme and LC ensures that H+ can be detected by the pH-sensitive LC before it is neutralized by buffer. Thus, the presence of OPs can be reported as color change in LC when they undergo enzymatic hydrolysis. For paraoxon detection, we can reach a limit of detection (LOD) of 1 μM by using the naked eye. This type of LC-based biosensor also shows high specificity and does not respond to imidacloprid and ampicillin.
關鍵字Organophosphate;Biosensors;Liquid crystal;Paraoxonase 1
語言英文
ISSN0925-4005;1873-3077
期刊性質國外
收錄於SCI;
產學合作
通訊作者Yang, Kun-lin
審稿制度
國別荷蘭
公開徵稿
出版型式,電子版,紙本
相關連結
Google+ 推薦功能,讓全世界都能看到您的推薦!