教師資料查詢 | 類別: 會議論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:Bioconversion of Shrimp Shell Wastes for Protease Production by a Newly Isolated Strain Chryseobacterium Taeanense TKU001
學年95
學期1
發表日期2007/05/25
作品名稱Bioconversion of Shrimp Shell Wastes for Protease Production by a Newly Isolated Strain Chryseobacterium Taeanense TKU001
作品名稱(其他語言)
著者王三郎; Wang, San-Lang; Yang, Chun-Hsiang; Liang, Tzu-Wen; Yen, Yue-Horng
作品所屬單位淡江大學生命科學研究所
出版者
會議名稱2007年 台灣幾丁質幾丁聚醣研討會=2007 Taiwan Chitin and Chitosan Symposium
會議地點中壢, 臺灣
摘要An extracellular metalloprotease with novel properties of solvent- and surfactant-stable was purified from the culture supernatant of Chryseobacterium taeanense TKU001 with shrimp shell wastes as the sole carbon/nitrogen source. Two extracellular proteases (FI and FII) were purified and characterized, and their molecular weights, pH and thermal stabilities were determined. The molecular masses of TKU001 protease FI and FII determined by SDS-PAGE and gel filtration were approximately 41 kDa and 75 kDa, respectively. TKU001 protease FI and FII were both inhibited completely by EDTA, indicating that the TKU001 protease FI and FII were metalloproteases. TKU001 protease FI and FII retained more than 75% of its original protease activity after preincubation for 10 days at 4.degree.C in the presence of 25% most tested organic solvents. The novelties of the TKU001 protease include its high stability to the solvents and surfactants. These unique properties make it an ideal choice for application in detergent formulations and enzymatic peptide synthesis.
關鍵字
語言中文
收錄於
會議性質國內
校內研討會地點
研討會時間20070525~20070525
通訊作者
國別中華民國
公開徵稿
出版型式
出處2007年 台灣幾丁質幾丁聚醣研討會論文集=Proceedings of 2007 Taiwan Chitin and Chitosan Symposium, 3p.
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