教師資料查詢 | 類別: 會議論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:Bioconversion of Shellfish Chitin Wastes for the Production of Bacillus subtilis W-118 Chitinase
學年95
學期1
發表日期2007/05/25
作品名稱Bioconversion of Shellfish Chitin Wastes for the Production of Bacillus subtilis W-118 Chitinase
作品名稱(其他語言)
著者王三郎; Wang, San-Lang; Lin, Tzu-Yin; Yen, Yue-Horng
作品所屬單位淡江大學生命科學研究所
出版者
會議名稱2007年 台灣幾丁質幾丁聚醣研討會=2007 Taiwan Chitin and Chitosan Symposium
會議地點中壢, 臺灣
摘要Bacillus subtilis W-118, an antifungal materials producing strain, excreted one chitinase when cultured in a medium containing shrimp and crab shell powder as major carbon source. The chitinase, purified by sequential chromatography, had a molecular mass of 20,600 and pI of 6. The optimum pH, optimum temperature, and pH stability of the chitinase were pH 6, 37.degree.C, and pH 5-7, respectively. The unique characteristics of the purified chitinase include low molecular mass and acidic pI. In the investigation of the inhibitory activity, it was found that the growth of F. oxysporum was 100% inhibited after incubation for 1 day with sterilized W-118 chitinase solution (5.6 units/mL).The chitinase hydrolyzates of chitin with low degrees of polymerization (DP) from 1 to 6 were analyzed by HPLC. Longer reaction time led to the generation of chitin oligosaccharides with lower DP. The chitin oligosaccharides were examined for their inhibitory effects on Fusarium oxysporum and human leukemia cell lines.
關鍵字
語言中文
收錄於
會議性質國內
校內研討會地點
研討會時間20070525~20070525
通訊作者
國別中華民國
公開徵稿
出版型式
出處2007年 台灣幾丁質幾丁聚醣研討會論文集=Proceedings of 2007 Taiwan Chitin and Chitosan Symposium, 2p.
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