教師資料查詢 | 類別: 會議論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:The Antitumor Activity of the Hydrolysates of Chitinous Materials Hydrolyzed by Crude Enzyme from Bacillus amyloliquefaciens V656
學年95
學期1
發表日期2007/05/25
作品名稱The Antitumor Activity of the Hydrolysates of Chitinous Materials Hydrolyzed by Crude Enzyme from Bacillus amyloliquefaciens V656
作品名稱(其他語言)
著者王三郎; Wang, San-Lang; Liang, Tzu-Wen; Chen, Yu-Jen; Yen, Yue-Horng
作品所屬單位淡江大學生命科學研究所
出版者
會議名稱2007年 台灣幾丁質幾丁聚醣研討會=2007 Taiwan Chitin and Chitosan Symposium
會議地點中壢, 臺灣
摘要Chitin, colloidal chitin and water-soluble chitosan were hydrolyzed by crude enzyme solution produce by Bacillus amyloliquefaciens V656. The hydrolysates with 12 h hydrolysis contained optimal (GlcNAc)6 and showed higher antitumor activity. Among those chitinous materials, the most effective one was the hydrolysates of water-soluble chitosan, which inhibited the growth of CT26 cells and reduced the survival rate to 34% in 1 day. Since the hydrolysate of water-soluble chitosan contained the optimal hexamer/(GlcNAc)6 at 12 h, it is conjectured that the antitumor activity should be related to (GlcNAc)6. This conjecture was further affirmed by experiment with pure (GlcNAc)6. However, This phenomenon might be due to the synergistic effect of the oligomers (GlcNAc)n, n = 1–6 in the hydrolysates. The antitumor effect of the chitinous hydrolysates is worth further investigation. The aim of this study was to investigate the induced apoptosis in CT26 cells by the hydrolysates of chitinous materials. It was found that the hydrolysates (A, B and C) inhibited the survival of CT26 cells in a concentrationand time-dependent manner. The hydrolysates induced characteristic DNA fragmentation of the CT26 cells. These results suggested that the hydrolysates from chitinous materials are potent apoptosisinducing agents for CT26 cells.
關鍵字
語言中文
收錄於
會議性質國內
校內研討會地點
研討會時間20070525~20070525
通訊作者
國別中華民國
公開徵稿
出版型式
出處2007年 台灣幾丁質幾丁聚醣研討會論文集=Proceedings of 2007 Taiwan Chitin and Chitosan Symposium, 3p.
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