教師資料查詢 | 類別: 期刊論文 | 教師: 梁慈雯 TZU-WEN LIANG (瀏覽個人網頁)

標題:Conversion of squid pen by Serratia ureilytica for the production of enzymes and antioxidants
學年97
學期1
出版(發表)日期2009/01/01
作品名稱Conversion of squid pen by Serratia ureilytica for the production of enzymes and antioxidants
作品名稱(其他語言)
著者Wang, San-lang; Lin, Chin-lin; Liang, Tzu-wen; Liu, Kao-cheng; Kuo, Yi-hsuan
單位淡江大學化學學系
出版者Amsterdam: Elsevier BV
著錄名稱、卷期、頁數Bioresource Technology 100(1), pp.316-323
摘要Two proteases (P1 and P2) and a chitinase (C1) were purified from the culture supernatant of Serratia ureilytica TKU013 with squid pen as the sole carbon/nitrogen source. The molecular masses of P1, P2 and C1 determined by SDS–PAGE were approximately 50 kDa, 50 kDa and 60 kDa, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of P1, P2 and C1 were (pH 10, 40 °C, pH 7–11, and <50 °C), (pH 10, 40 °C, pH 8–11, and <40 °C) and (pH 6, 50 °C, pH 5–8, and <50 °C), respectively. P1 and P2 were inhibited by Mg2+, EDTA and C1 was inhibited completely by Cu2+. The antioxidant activity of TKU013 culture supernatant was 72% per mL (DPPH scavenging ability). With this method, we have shown that squid pen wastes can be utilized and have revealed its hidden potential in the production of functional foods.
關鍵字Serratia ureilytica; Chitinase; Protease; Antioxidant activity; Squid pen wastes
語言英文
ISSN0960-8524
期刊性質國外
收錄於SCI;EI
產學合作
通訊作者Wang, San-lang
審稿制度
國別荷蘭
公開徵稿
出版型式紙本
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