教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:Conversion of shrimp shell by using Serratia sp. TKU017 fermentation for the production of enzymes and antioxidants
學年98
學期1
出版(發表)日期2010/01/01
作品名稱Conversion of shrimp shell by using Serratia sp. TKU017 fermentation for the production of enzymes and antioxidants
作品名稱(其他語言)
著者Wang, San-Lang; Li, J.Y.; Liang, Tzu-Wen; Hsieh, Jia-Lin; Tseng, Wan-Nine
單位淡江大學生命科學研究所
出版者Seoul: HHan'gug Mi'saengmul Saengmyeong Gong Haghoe
著錄名稱、卷期、頁數Journal of Microbiology and Biotechnology 20(1), pp.117-126
摘要A chitinase (CHT), and a protease (PRO) were purified from the culture supernatant of Serratia sp. TKU017 with shrimp shell as the sole carbon/nitrogen source. The molecular masses of CHT and PRO determined by SDS-PAGE were approximately 65 kDa and 53 kDa, respectively. CHT was inhibited by Mn2+, Cu2+ and PRO was inhibited by most tested divalent metals, EDTA. The optimum pH, optimum temperature, pH stability, and thermal stability of CHT and PRO were (pH 5, 50°C, pH 5–7, <50°C) and (pH 9, 40°C, pH 5–11, <40°C), respectively. PRO retained 95% of its protease activity in the presence of 0.5 mM SDS. The result demonstrates that PRO is SDS-resistant protease and probably has a rigid structure. The 4th day supernatant showed the strongest antioxidant activity (70%, DPPH scavenging ability) and the highest total phenolic content (196±6.2 μg of gallic acid equival/mL). Significant associations between the antioxidant potency and the total phenolic content, as well as between the antioxidant potency and free amino groups, were found for the supernatant. With this method, we have shown that shrimp shell wastes can be utilized and it’s effective in the production of enzymes and antioxidants, facilitating its potential use in industrial applications and functional foods.
關鍵字chitinase;protease;antioxidant activity;Serratia sp.;shrimp shell wastes
語言英文
ISSN1017-7825
期刊性質國外
收錄於SCI
產學合作
通訊作者Wang, San-Lang
審稿制度
國別韓國
公開徵稿
出版型式紙本
相關連結
Google+ 推薦功能,讓全世界都能看到您的推薦!