教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:A novel nattokinase produced by Pseudomonas sp. TKU015 using shrimp shells as substrate
學年97
學期1
出版(發表)日期2009/01/01
作品名稱A novel nattokinase produced by Pseudomonas sp. TKU015 using shrimp shells as substrate
作品名稱(其他語言)
著者Wang, San-Lang; Chen, Hsin-Jen; Liang, Tzu-Wen; Lin, Yung-Di
單位淡江大學生命科學研究所
出版者Camden: Elsevier Ltd
著錄名稱、卷期、頁數Process Biochemistry 44(1), pp.70-76
摘要A nattokinase was purified from the culture supernatant of Pseudomonas sp. TKU015 with shrimp shell wastes as the sole carbon/nitrogen source. The molecular masses of TKU015 nattokinase determined by SDS-PAGE and gel filtration were approximately 21 and 24 kDa, respectively. The optimum pH, optimum temperature, pH stability, and thermal stability of TKU015 nattokinase were 7, 50 °C, pH 4–11, and less than 50 °C, respectively. TKU015 nattokinase was inhibited completely by PMSF, indicating that the TKU015 nattokinase was serine protease. The results of peptide mass mapping showed that two tryptic peptides of the nattokinase were identical to a chitin binding protein from Bacillus cereus ATCC 14579 (GenBank accession number gi30020946) with 23% sequence coverage. With this method, Pseudomonas sp. TKU015 produces a nattokinase/fibrinolytic enzyme and may be considered as a new source for thrombolytic agents.
關鍵字Fibrinolytic enzyme; Nattokinase; Pseudomonas sp.; Shrimp shell wastes
語言英文
ISSN1359-5113
期刊性質國外
收錄於SCI;EI
產學合作
通訊作者Wang, San-Lang
審稿制度
國別美國
公開徵稿
出版型式紙本
相關連結
Google+ 推薦功能,讓全世界都能看到您的推薦!