教師資料查詢 | 類別: 期刊論文 | 教師: 王三郎 WANG SAN-LANG (瀏覽個人網頁)

標題:Degradation of chitin and production of bioactive materials by bioconversion of squid pens
學年98
學期1
出版(發表)日期2009/09/01
作品名稱Degradation of chitin and production of bioactive materials by bioconversion of squid pens
作品名稱(其他語言)
著者Wang, San-Lang; Yang, Chiann-Wen; Liang, Tzu-Wen; Peng, Juo-Hua; Wang, Chuan-Lu
單位淡江大學化學學系
出版者Kidlington: Pergamon
著錄名稱、卷期、頁數Carbohydrate Polymers 78(2), pp.205-212
摘要Serratia marcescens TKU011, a protease- and chitosanase-producing bacterium, the optimized condition for protease and chitosanase production was found after the media were heated at 121 °C for 120 min and the culture was shaken at 25 °C for 5 days in 100 mL of medium containing 1% squid pen powder (SPP) (w/v), 0.1% K2HPO4, and 0.05% MgSO4. An extracellular metalloprotease with novel properties of solvent stable, and alkaline was purified from the culture supernatant of S. marcescens TKU011 with squid pen wastes as the sole carbon/nitrogen source. The enzyme was a monomeric protease with a molecular mass of 48–50 kDa by SDS–PAGE and gel filtration chromatography. The optimum pH, optimum temperature, pH stability, and thermal stability of TKU011 protease were 8, 50 °C, pH 5–11, and <40 °C, respectively. Besides protease and chitosanase, with this method, deproteinization of squid pen for β-chitin, the production of peptide and reducing sugar may be useful for biological applications.
關鍵字Serratia marcescens; Protease; Chitosanase; Squid pen wastes; Deproteinization
語言英文
ISSN0144-8617
期刊性質國外
收錄於SCI
產學合作
通訊作者
審稿制度
國別英國
公開徵稿
出版型式
相關連結
Google+ 推薦功能,讓全世界都能看到您的推薦!