|標題：Biodegradation of Poly(ß-hydroxybutyrate) by a novel isolate of Streptomyces bangladeshensis 77T-4|
|作品名稱||Biodegradation of Poly(ß-hydroxybutyrate) by a novel isolate of Streptomyces bangladeshensis 77T-4|
|著者||Ke-Jen Hsu; Min Tseng; Don, Trong-Ming; Mei-Kwei Yang|
|出版者||Taipei: Academia Sinica * Institute of Plant and Microbial Biology|
|著錄名稱、卷期、頁數||Botanical Studies 53(3), pp.307-313|
|摘要||A thermophilic Streptomyces bangladeshensis isolate designated 77T-4 that can degrade
poly(β-hydroxybutyrate) (PHB) was isolated from a soil sample in Taiwan. Isolate 77T-4 grew well in ureafructose oatmeal medium and produced a clear zone around the colony when grown on an agar plate containing emulsified PHB, indicating the presence of an extracellular PHB depolymerases. A PHB-degrading enzyme was purified to homogeneity from the culture supernatant. The molecular weight of the PHB-degrading enzyme was estimated to be approximately 40 kDa. A small portion of the N-terminal sequence (Ala-Val-Pro-Leu-Thr-Arg ) of the purified enzyme was determined and found to share a significant homology with that of the PHB depolymerase of Streptomyces sp. MG.