教師資料查詢 | 類別: 期刊論文 | 教師: 陳曜鴻 CHEN, YAU-HUNG (瀏覽個人網頁)

標題:Caffeine treatment disturbs the angiogenesis of zebrafish embryos
學年101
學期1
出版(發表)日期2012/10/01
作品名稱Caffeine treatment disturbs the angiogenesis of zebrafish embryos
作品名稱(其他語言)
著者Yeh, Chien-Hung; Liao, Yun-Feng; Chang, Chao-Yuan; Tsai, Jen-Ning; Wang, Yun-Hsin; Cheng, Chien-Chung; Wen, Chi-Chung; Chen, Yau-Hung
單位淡江大學生命科學研究所; 淡江大學化學學系
出版者New York: Informa Healthcare
著錄名稱、卷期、頁數Drug and Chemical Toxicology 35(4), pp.361-365
摘要Caffeine is a widely consumed substance that occurs in numerous dietary sources, but teratogenic effects of caffeine intake during embryonic development are still not clear. In the present study, we used the zebrafish as a model to assess caffeine-induced toxicity on embryonic vascular development. A green fluorescent vascular endothelium transgenic line, Tg(fli1:egfp), was utilized for the sensitive detection of vascular development, including vasculo- and angiogenesis. Caffeine-treated embryos showed no defects in vasculogenesis, but revealed dose-dependent (250-350 ppm) developmental defects in intersegmental vessels, dorsal longitudinal anastomotic vessels, and subintestinal vein sprouting. Further, real-time polymerase chain reaction analysis of caffeine-treated embryos showed an upregulation of nrp1a along with a downregulation of sema3aa and sema3c. In conclusion, caffeine treatment induces defects of angiogenesis in zebrafish embryos.
關鍵字Angiogenesis;caffeine;fluorescent protein;transgenic fish;zebrafish
語言英文(美國)
ISSN0148-0545
期刊性質國外
收錄於SCI
產學合作
通訊作者Chen, Yau-Hung
審稿制度
國別美國
公開徵稿
出版型式
相關連結
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