Identification of Paenibacillus sp. 2S-6 and application of its xylanase on biobleaching
學年 99
學期 2
出版（發表）日期 2011-03-01
作品名稱 Identification of Paenibacillus sp. 2S-6 and application of its xylanase on biobleaching
作品名稱（其他語言）
著者 Ko, Chun-han; Tsai, Chung-hung; Tu, Jenn; Yang, Bing-yuan; Hsieh, Dong-lin; Jane, Wann-neng; Shih, Tzenge-lien
單位 淡江大學化學學系
出版者 London: Elsevier Ltd
著錄名稱、卷期、頁數 International Biodeterioration and Biodegradation 65(2), pp.334-339
摘要 A Paenibacillus sp. strain 2S-6 was isolated from the black liquor of the first brownstock washing stage of kraft pulping process and identified by its 16S rDNA sequence. This bacterial strain utilized a variety of saccharides and polysaccharides as carbon source, but neither lignin nor lipids. Crude xylanase from Paenibacillus sp. 2S-6 was produced in a 5 L laboratory fermenter at 37 °C, pH 7. After 24 h, up to 10.5 IU xylanase per mg of protein in the crude extract of fermentation broth was obtained. After two-stage ultrafiltration, the optimal activity of partially purified xylanase reached 60.51 IU/mg at 50 °C, pH 6. A major band indicating molecular weight of 33 kDa was shown on SDS-PAGE for the partially purified xylanase. After 4 h at 60 °C, 48.99% and 31.25% residual xylanase activities were demonstrated at pH 7 and 9, respectively. Efficacy of its xylanase on the bleaching agent saving was demonstrated by using 5 IU xylanase per gram oven-dried pulp prior to bleaching, referred as biobleaching. Identical levels of brightness and higher levels of viscosity were obtained for the xylanase pretreated eucalypt kraft pulps followed by a 20% reduction of the bleaching agent dosage in the first step of a commercial C70/D30-Eo-D bleaching sequence.
關鍵字 Biobleaching; Bleaching pretreatment; Eucalypt; Kraft pulp; Paenibacillus; Xylanase
語言 en
ISSN 0964-8305
期刊性質 國外
收錄於 SCI EI
產學合作
通訊作者 Ko, Chun-han
審稿制度
國別 GBR
公開徵稿
出版型式 紙本
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