教師資料查詢 | 類別: 期刊論文 | 教師: 林達鎔 LIN, DAR-JONG (瀏覽個人網頁)

標題:Surface modification of microporous PVDF membranes for neuron culture
學年98
學期2
出版(發表)日期2010/03/01
作品名稱Surface modification of microporous PVDF membranes for neuron culture
作品名稱(其他語言)
著者Young, Tai-Horng; Chang, Hsu-Hsien; Lin, Dar-Jong; Cheng, Liao-Ping
單位淡江大學化學工程與材料工程學系
出版者Amsterdam: Elsevier BV
著錄名稱、卷期、頁數Journal of Membrane Science 350(1-2), pp.32-41
摘要Microporous poly(vinylidene fluoride) (PVDF) membranes with either dense or porous surface were prepared by isothermal immersion–precipitation of a casting solution in coagulation baths of different strengths. Onto the membranesurface, amino acid (l-lysine) or 1,6-hexanediamine (HMDA) was chemically immobilized. The membrane was first grafted with poly(glycidyl methacrylate) (PGMA) by means of plasma-induced free radical polymerization. Then, l-lysine or 1,6-hexanediamine was reacted with epoxy groups in the grafted PGMA to create an ECM environment suited to cell culture. Neuronal cells were cultivated on the formed pristine and surface-modified PVDFmembranes. It is found that neurons tend to aggregate into large clusters with neuritic branches on pristine PVDFmembranes, whereas for those cultured on the PGMA/PVDFmembranes, serious aggregation takes place as well, but without neurites being formed. In contrast, neurons did not aggregate on the l-lysine or HMDA-immobilized membrane. It is also interesting to find that a neuritic network inter-connecting cells is constructed for the former, yet no neurite is observed for the latter membrane. Such phenomenon is thought to be associated with the extra –COOH group in l-lysine with respect to HMDA.
關鍵字Poly(vinylidene fluoride); Porous membrane; Immobilization; l-lysine; Neuron culture
語言英文
ISSN0376-7388
期刊性質國外
收錄於SCI
產學合作
通訊作者
審稿制度
國別荷蘭
公開徵稿
出版型式紙本
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