教師資料查詢 | 類別: 期刊論文 | 教師: 陳曜鴻 CHEN, YAU-HUNG (瀏覽個人網頁)

標題:Embryonic exposure to diclofenac disturbs actin organization and leads to myofibril misalignment
學年99
學期2
出版(發表)日期2011/04/01
作品名稱Embryonic exposure to diclofenac disturbs actin organization and leads to myofibril misalignment
作品名稱(其他語言)
著者Chen, Ying-hsin; Chang, Chao-yuan; Wang, Yun-hsin; Wen, Chi-chung; Chen, Yun-chen; Hu, Sheng-chuan; Yu, Dah-shyong; Chen, Yau-hung
單位淡江大學生命科學研究所; 淡江大學數學學系
出版者Hoboken: John Wiley & Sons, Inc.
著錄名稱、卷期、頁數Birth Defects Research Part B: Developmental and Reproductive Toxicology 92(2), pp.139-147
摘要The objective of this study was to investigate the embryotoxicity of diclofenac. Zebrafish (Danio rerio) embryos at 12 hpf were treated with different dosages of diclofenac (0–2,000 ppm) for different time courses (12–72 hr). Results showed no evident differences in survival rates or morphological changes between the mock-treated control (0 ppm) zebrafish embryos and those with 1-ppm diclofenac-exposure (12–24, 12–36 hpf). In contrast, after higher doses (5 and 10 ppm) of exposure, embryos displayed some defective phenotypes, including malformed somite boundary, a twisted body axis, and shorter body length. In addition, diclofenac-treated embryos exhibited significantly reduced frequencies of spontaneous in-chorion contractions in comparison with mock-control littermates (mock-control: 13.20±2.24 vs. 5–10 ppm diclofenac: 6.66±1.35–3.03±1.84). Subtle changes were easily observed by staining with specific monoclonal antibodies F59 and phalloidin to detect morphological changes in muscle fibers and formation of F-actin, respectively. Our data show that diclofenac treatment disturbs actin organization and muscle fiber alignment, thus causing malformed somite phenotypes.
關鍵字actin;diclofenac;myotoxic;myod;zebrafish
語言英文(美國)
ISSN1542-9733; 1542-9741
期刊性質國外
收錄於SCI
產學合作
通訊作者Chen, Yau-hung
審稿制度
國別美國
公開徵稿
出版型式紙本
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